Electrochemical Oxidization of Herring Sperm DNA and its Interaction with Histone

Authors

Juan XU, School of Chemistry and Environment,South China Normal University,Guangzhou 510006,China2Unit of Molecular Toxicology,Center for Disease Control and Prevention,Guangzhou 510080,China;
Gui-ping HUANG, School of Chemistry and Environment,South China Normal University,Guangzhou 510006,China2Unit of Molecular Toxicology,Center for Disease Control and Prevention,Guangzhou 510080,China;
Hong LI, School of Chemistry and Environment,South China Normal University,Guangzhou 510006,China2Unit of Molecular Toxicology,Center for Disease Control and Prevention,Guangzhou 510080,China;
Wei ZHU

Abstract

The voltammetric behavior of herring sperm DNA at glassy carbon electrode and its interaction with histone have been investigated by using cyclic voltammetry,differential pulse voltammetry,and fluorescence measurement.In the potential range of 0.20 V to 1.25 V,it is found that an irreversible oxidative peak is exhibited in acid solution,but in the neutral or alkaline solution two oxidative peaks are observed.Oxidative peak potential values show a negative shift with increasing pH via a slope of-57 mV·pH-1 and a linear relation with DNA concentration in the range of 0.45 to 8.20 mmol·L-1.In the presence of histone,the oxidative peak current of DNA and the fluorescence intensity of interactive product between a ruthenium complex and DNA are found to have an obvious decrease and the oxidative potentials show a positive shift.Moreover,the experimental results suggest that the DNA damage is weakened based on its binding interaction with histone.

Keywords

herring sperm DNA, electrochemical oxidation, histone, interaction

DOI Link

https://doi.org/10.61558/2993-074X.1836

Publication Date

2007-11-28

Online Available Date

2007-11-28

Revised Date

2007-11-28

Received Date

2007-11-28

Recommended Citation

Juan XU, Gui-ping HUANG, Hong LI, Wei ZHU. Electrochemical Oxidization of Herring Sperm DNA and its Interaction with Histone[J]. Journal of Electrochemistry, 2007 , 13(4): 372-376.
DOI: 10.61558/2993-074X.1836
Available at: https://jelectrochem.xmu.edu.cn/journal/vol13/iss4/3

References

[1]Qin S,Parthun M R.Histone H3 and the histoneacetyltransferase Hat1p contribute to DNA double-strand break repair[J].Mol Cell Biol,2002,22(23):8353-8365.
[2]Daniel D C,Thompson M,Woodbury N W.Fluores-cence intensity fluctuations of individual labeled DNAfragments and a DNA binding protein in solution at thesingle molecule level:A comparison of photobleaching,diffusion,and binding dynamics[J].J Phys Chem B,2000,104(6):1382-1390.
[3]Tachi-iri Y,Ishikawa M,Hirano K.Investigation of thehydrolysis of single DNA molecules using fluorescencevideo microscopy[J].Anal Chem,2000,72(7):1649-1656.
[4]Guo Z X,Li L,Shen H X,et al.Bromopyrogallol redenhanced resonance light-scattering spectroscopic deter-mination of DNA with 5,10,15,20-tetrakis[4-(trime-thylammoniumyl)phenyl]porphine[J].Anal Chim Ac-ta,1999,379(1~2):45-51.
[5]Yoburn J C,Deb S,Manfield I W,et al.Abiaryl pep-tide crosslink in a MetJ peptide model confers coopera-tive,nonspecific binding to DNA that ablates both re-pressor binding and in vitro transcription[J].BioorgMed Chem,2003,11(6):811-816.
[6]Hao D Y,Ohme-Takagi M,Yamasaki K.A modifiedsensor chip for surface plasmon resonance enables a rap-id determination of sequence specificity of DNA-bindingproteins[J].FEBS Lett,2003,536(1~3):151-156.
[7]Obrien J C,Stickney J T,Porter M D.Self-assembleddouble-stranded DNA(dsDNA)microarrays for pro-tein:dsDNA screening using atomic force microscopy[J].J Am Chem Soc,2000,122(20):5004-5005.
[8]Seong G H,Niimi T,Yanagida Y,et al.Single-molec-ular AFMprobing of specific DNAsequencing using Re-cA-promoted homologous pairing and strand exchange[J].Anal Chem,2000,72(6):1288-1293.
[9]Lu MM,Zhang J G.Mass spectrometry and its applica-tion in life sciences[J].Biotech Inform,2001,(4):38-41.
[10]Zhou A H,Xie Q J,Li P,et al.Piezoelectric crystalimpedance analysis for investigating the modificationprocesses of protein,cross-linker,and DNA on goldsurface[J].Appl Surf Sci,2000,158(1~2):141-146.
[11]Takeuchi A,Sode K.A Salmonella detection systemusing an engineered DNA binding protein that specific-ally captured a DNAsequence[J].Anal Chem,2000,72(13):2809-2813.
[12]Makhatadze G I,Loladze V V,Gribenko A V,et al.Mechanism of thermostabilization in a designed coldshock protein with optimized surface electrostatic inter-actions[J].J Mol Biol,2004,336(4):929-942.
[13]Kato R,Kataoka M,Kamikubo H,et al.Direct obser-vation of three conformations of MutS protein regulatedby adenine nucleotides[J].J Mol Biol,2001,309(1):227-238.
[14]Brewer S H,Cresawn S G,Nguyen D T,et al.Differ-ence FT-IR studies of nucleotide binding to the recom-bination protein RecA[J].J Phys Chem B,2000,104(29):6950-6954.
[15]Elstner M,Frauenheim T,Suhai S.An approximateDFT method for QM/MM simulations of biologicalstructures and processes[J].J Mol Struct(Theo-chem),2003,632(1~3):29-41.
[16]Nassar A E F,Rusling J F,Nakashima N.Electrontransfer between electrodes and heme proteins in pro-tein-DNA films[J].J Am Chem Soc,1996,18(12):3043-3044.
[17]Lvov Y M,Lu Z Q,Schenkman J B,et al.Direct e-lectrochemistry of myoglobin and cytochrome P450camin alternate layer-by-layer films with DNA and otherpolyions[J].J Am Chem Soc,1998,120(17):4073-4080.
[18]Lu T,Qu X,Dong X,et al.The direct electrochemis-try of cytochrome c at the nanometer-sized rare earthelement oxide particle-modified gold electrodes[J].JMol Catal A:Chemical,1996,106(1~2):1-5.
[19]Valetti F,Sadeghi S J,Meharenna Y T,et al.Engi-neering multi-domain redox proteins containing fla-vodoxin as bio-transformer:preparatory studies by ra-tional design[J].Biosens Bioelectron,1998,13(6):675-685.
[20]Chen X H,Ruan C M,Kong J L,et al.Characteriza-tion of the direct electron transfer and bioelectrocatalys-is of horseradish peroxidase in DNA film at pyrolyticgraphite electrode[J].Anal Chim Acta,2000,412(1~2):89-98.
[21]Lin L,Li J R,Jiang L.Fixation of single-strandedDNA nucleotide by self assembly[J].Colloids Surf A:Physicochem Eng Aspects,2000,175(1~2):11-15.
[22]Wang Z X,Liu D J,Dong S J.In situ infrared spec-troelectrochemical studies on adsorption and oxidationof nucleic acids at glassy carbon electrode[J].Bioel-ectrochem Bioenerg,2001,53(2):175-181.
[23]Rusling J F.Sensors for toxicity of chemicals and oxi-dative stress based on electrochemical catalytic DNAoxidation[J].Biosens Bioelectron,2004,20(5):1022-1028.
[24]Zhen Q X(甄启雄),Ye B H(叶保辉),Liu J G(刘劲刚),et al.Synthesis of ruthenium polypyridyl com-plexes and the steric influence of the intercalated lig-and on DNA-binding[J].Chem J Chinese Univer,1999,20(11):1661-1666.