An Electrochemiluminescence-Based Arsenic (III) Sensor using Luminol on Screen-Printed Gold Electrodes

Authors

Harmesa Harmesa, Department of Chemistry, Faculty of Mathematics and Natural Science, Universitas Indonesia, Depok, 16424, Indonesia; Research Center for Oceanology, National Research and Innovation Agency, BJ. Habibie S&T Complex, South Tangerang, Banten, 15310, Indonesia.
Isnaini Rahmawati, Department of Chemistry, Faculty of Mathematics and Natural Science, Universitas Indonesia, Depok, 16424, Indonesia.
Andrea Fiorani, Department of Chemistry, Keio University, 3-14-1 Hiyoshi, Yokohama, Kanagawa 223-8522, Japan.
Yasuaki Einaga, Department of Chemistry, Keio University, 3-14-1 Hiyoshi, Yokohama, Kanagawa 223-8522, Japan.
Eny Kusrini, Department of Chemical Engineering, Universitas Indonesia, Depok, 16424, Indonesia.
A'an Johan Wahyudi, Research Center for Oceanology, National Research and Innovation Agency, BJ. Habibie S&T Complex, South Tangerang, Banten, 15310, Indonesia.
Asep Saefumillah, Department of Chemistry, Faculty of Mathematics and Natural Science, Universitas Indonesia, Depok, 16424, Indonesia.
Tribidasari A. Ivandini, Department of Chemistry, Faculty of Mathematics and Natural Science, Universitas Indonesia, Depok, 16424, Indonesia.Follow

Document Type

Article

Corresponding Author(s)

Tribidasari A. Ivandini(ivandini.tri@sci.ui.ac.id)

Abstract

Electrochemiluminescence (ECL) of luminol has been studied on a screen-printed gold electrode for a simple and sensitive detection of arsenic ions (As(III)). Cyclic voltammetry (CV) was applied as the proposed technique to study luminol's electrochemical behavior and to evaluate the arsenic's effect in the ECL system, while hydrogen peroxide served as a co-reactant to enhance luminol’s light emission under alkaline conditions. To achieve optimal electrode performance, key parameters including pH, scan rate, and the concentrations of H₂O₂ and luminol were carefully optimized. The presence of As(III) induced a quenching effect on the luminol/H₂O₂ ECL system, leading to a linear decrease in ECL signal across the wide concentration range of 1 nM to 150 µM. The system demonstrated a low detection limit of 1.21 nM and exhibited excellent repeatability with a relative standard deviation of 2.27%, highlighting its sensitivity and reliability for As(III) detection. A key advantage of this study was the successful use of commercial bare electrodes, which were readily available and required no modifications, proving their effectiveness for ECL-based arsenic sensing. The optimized buffer solution pH of 10 played a critical role in enhancing arsenic detection selectivity, as it facilitated the optimal deprotonation of luminol and ensured arsenic remained in its dissolved state, whereas other potential metal ion interferences were more likely to form solid metal (hydro)oxides. Furthermore, the developed sensor was successfully applied for As(III) detection in a seawater matrix, demonstrating its potential as a robust and effective ECL-based arsenic sensor for environmental applications.

Graphical Abstract

Keywords

As(III), Electrochemiluminescence, Gold electrode, H2O2, Quenching effect

DOI

10.61558/2993-074X.3593

Online Date

11-14-2025

Recommended Citation

Harmesa Harmesa, Isnaini Rahmawati, Andrea Fiorani, Yasuaki Einaga, Eny Kusrini, A'an Johan Wahyudi, Asep Saefumillah, Tribidasari A. Ivandini. An Electrochemiluminescence-Based Arsenic (III) Sensor using Luminol on Screen-Printed Gold Electrodes[J]. Journal of Electrochemistry, doi: 10.61558/2993-074X.3593.