Platinum Nanoparticle-Based Collision Electrochemistry for Rapid Detection of Breast Cancer MCF-7 Cells

Fu-Xing Qin, Hubei Key Laboratory for Precision Synthesis of Small Molecule Pharmaceuticals & Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules & College of Chemistry and Chemical Engineering, Hubei University, Wuhan, 430062, P. R. China
Ming-Ke Li, Hubei Key Laboratory for Precision Synthesis of Small Molecule Pharmaceuticals & Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules & College of Chemistry and Chemical Engineering, Hubei University, Wuhan, 430062, P. R. China
Hui-Long Zhou, Hubei Key Laboratory for Precision Synthesis of Small Molecule Pharmaceuticals & Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules & College of Chemistry and Chemical Engineering, Hubei University, Wuhan, 430062, P. R. China
Wei Wen, Hubei Key Laboratory for Precision Synthesis of Small Molecule Pharmaceuticals & Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules & College of Chemistry and Chemical Engineering, Hubei University, Wuhan, 430062, P. R. China
Xiu-Hua Zhang, Hubei Key Laboratory for Precision Synthesis of Small Molecule Pharmaceuticals & Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules & College of Chemistry and Chemical Engineering, Hubei University, Wuhan, 430062, P. R. China
Sheng-Fu Wang, Hubei Key Laboratory for Precision Synthesis of Small Molecule Pharmaceuticals & Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules & College of Chemistry and Chemical Engineering, Hubei University, Wuhan, 430062, P. R. China
Zhen Wu, Hubei Key Laboratory for Precision Synthesis of Small Molecule Pharmaceuticals & Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules & College of Chemistry and Chemical Engineering, Hubei University, Wuhan, 430062, P. R. ChinaFollow

Document Type

Article

Corresponding Author(s)

Zhen Wu(wuzhen@hubu.edu.cn)

Abstract

Cancer metastasis is the leading cause of death in cancer patients worldwide and one of the major challenges in treating cancer. Circulating tumor cells (CTCs) play a pivotal role in cancer metastasis. However, the content of CTCs in peripheral blood is minimal, so the detection of CTCs in real samples is extremely challenging. Therefore, efficient enrichment and early detection of CTCs are essential to achieve timely diagnosis of diseases. In this work, we constructed an innovative and sensitive single-nanoparticle collision electrochemistry (SNCE) biosensor for the detection of MCF-7 cells (human breast cancer cells) by immunomagnetic separation technique and liposome signal amplification strategy. Liposomes embedded with platinum nanoparticles (Pt NPs) were used as signal probes, and homemade gold ultramicroelectrodes (Au UME) were used as working electrodes. The effective collision between Pt NPs and UME would produce distinguishable step-type current. MCF-7 cells were accurately quantified according to the relationship between cell concentration and collision frequency (the number of step-type currents generated per unit time), realizing highly sensitive and specific detection of MCF-7 cells. The SNCE biosensor has a linear range of 10 cells/mL to 105 cells/mL with a detection limit as low as 5 cells/mL. In addition, the successful detection of MCF-7 cells in complex samples showed that SNCE biosensors have great potential for patient sample detection.

Graphical Abstract

Keywords

Circulating tumor cells, Single-nanoparticle collision electrochemistry, Immunomagnetic separation, Liposome, Platinum nanoparticles

Online Date

6-9-2024

Supporting Information
2414004SI.pdf (1565 kB)
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