Authors
Fuxing Qin, Hubei Key Laboratory for Precision Synthesis of Small Molecule Pharmaceuticals & Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules & College of Chemistry and Chemical Engineering, Hubei University, Wuhan, 430062, P. R. China
Mingke Li, Hubei Key Laboratory for Precision Synthesis of Small Molecule Pharmaceuticals & Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules & College of Chemistry and Chemical Engineering, Hubei University, Wuhan, 430062, P. R. China
Huilong Zhou, Hubei Key Laboratory for Precision Synthesis of Small Molecule Pharmaceuticals & Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules & College of Chemistry and Chemical Engineering, Hubei University, Wuhan, 430062, P. R. China
Wei Wen, Hubei Key Laboratory for Precision Synthesis of Small Molecule Pharmaceuticals & Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules & College of Chemistry and Chemical Engineering, Hubei University, Wuhan, 430062, P. R. China
Xiuhua Zhang, Hubei Key Laboratory for Precision Synthesis of Small Molecule Pharmaceuticals & Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules & College of Chemistry and Chemical Engineering, Hubei University, Wuhan, 430062, P. R. China
Shengfu Wang, Hubei Key Laboratory for Precision Synthesis of Small Molecule Pharmaceuticals & Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules & College of Chemistry and Chemical Engineering, Hubei University, Wuhan, 430062, P. R. China
Zhen Wu, Hubei Key Laboratory for Precision Synthesis of Small Molecule Pharmaceuticals & Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules & College of Chemistry and Chemical Engineering, Hubei University, Wuhan, 430062, P. R. China
Corresponding Author(s)
Circulating tumor cells, Single-nanoparticle collision electrochemistry, Immunomagnetic separation, Liposome, Platinum nanoparticles
Abstract
Cancer metastasis is the leading cause of death in cancer patients worldwide and one of the major challenges in treating cancer. Circulating tumor cells (CTCs) play a pivotal role in cancer metastasis. However, the content of CTCs in peripheral blood is minimal, so the detection of CTCs in real samples is extremely challenging. Therefore, efficient enrichment and early detection of CTCs are essential to achieve timely diagnosis of diseases. In this work, we constructed an innovative and sensitive single-nanoparticle collision electrochemistry (SNCE) biosensor for the detection of MCF-7 cells (human breast cancer cells) by immunomagnetic separation technique and liposome signal amplification strategy. Liposomes embedded with platinum nanoparticles (Pt NPs) were used as signal probes, and homemade gold ultramicroelectrodes (Au UME) were used as working electrodes. The effective collision between Pt NPs and UME would produce distinguishable step-type current. MCF-7 cells were accurately quantified according to the relationship between cell concentration and collision frequency (the number of step-type currents generated per unit time), realizing highly sensitive and specific detection of MCF-7 cells. The SNCE biosensor has a linear range of 10 cells/mL to 105 cells/mL with a detection limit as low as 5 cells/mL. In addition, the successful detection of MCF-7 cells in complex samples showed that SNCE biosensors have great potential for patient sample detection.
Graphical Abstract
Keywords
Circulating tumor cells, Single-nanoparticle collision electrochemistry, Immunomagnetic separation, Liposome, Platinum nanoparticles
DOI
10.61558/2993-074X.3483
Recommended Citation
Fuxing Qin, Mingke Li, Huilong Zhou, Wei Wen, Xiuhua Zhang, Shengfu Wang, Zhen Wu . Platinum Nanoparticle-Based Collision Electrochemistry for Rapid Detection of Breast Cancer MCF-7 Cells[J]. Journal of Electrochemistry, doi: 10.61558/2993-074X.3483.
