Platinum Nanoparticle-Based Collision Electrochemistry for Rapid Detection of Breast Cancer MCF-7 Cells

Authors

Fu-Xing Qin, Hubei Key Laboratory for Precision Synthesis of Small Molecule Pharmaceuticals & Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules & College of Chemistry and Chemical Engineering, Hubei University, Wuhan, 430062, P. R. China
Ming-Ke Li, Hubei Key Laboratory for Precision Synthesis of Small Molecule Pharmaceuticals & Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules & College of Chemistry and Chemical Engineering, Hubei University, Wuhan, 430062, P. R. China
Hui-Long Zhou, Hubei Key Laboratory for Precision Synthesis of Small Molecule Pharmaceuticals & Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules & College of Chemistry and Chemical Engineering, Hubei University, Wuhan, 430062, P. R. China
Wei Wen, Hubei Key Laboratory for Precision Synthesis of Small Molecule Pharmaceuticals & Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules & College of Chemistry and Chemical Engineering, Hubei University, Wuhan, 430062, P. R. China
Xiu-Hua Zhang, Hubei Key Laboratory for Precision Synthesis of Small Molecule Pharmaceuticals & Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules & College of Chemistry and Chemical Engineering, Hubei University, Wuhan, 430062, P. R. China
Sheng-Fu Wang, Hubei Key Laboratory for Precision Synthesis of Small Molecule Pharmaceuticals & Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules & College of Chemistry and Chemical Engineering, Hubei University, Wuhan, 430062, P. R. China
Zhen Wu, Hubei Key Laboratory for Precision Synthesis of Small Molecule Pharmaceuticals & Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules & College of Chemistry and Chemical Engineering, Hubei University, Wuhan, 430062, P. R. ChinaFollow

Corresponding Author

Zhen Wu(wuzhen@hubu.edu.cn)

Abstract

Cancer metastasis is the leading cause of death in cancer patients worldwide and one of the major challenges in treating cancer. Circulating tumor cells (CTCs) play a pivotal role in cancer metastasis. However, the content of CTCs in peripheral blood is minimal, so the detection of CTCs in real samples is extremely challenging. Therefore, efficient enrichment and early detection of CTCs are essential to achieve timely diagnosis of diseases. In this work, we constructed an innovative and sensitive single-nanoparticle collision electrochemistry (SNCE) biosensor for the detection of MCF-7 cells (human breast cancer cells) by immunomagnetic separation technique and liposome signal amplification strategy. Liposomes embedded with platinum nanoparticles (Pt NPs) were used as signal probes, and homemade gold ultramicroelectrodes (Au UME) were used as the working electrodes. The effective collision between Pt NPs and UME would produce distinguishable step-type current. MCF-7 cells were accurately quantified according to the relationship between cell concentration and collision frequency (the number of step-type currents generated per unit time), realizing highly sensitive and specific detection of MCF-7 cells. The SNCE biosensor has a linear range of 10 cells·mL^–1 to 10⁵ cells·mL^–1 with a detection limit as low as 5 cells·mL^–1. In addition, the successful detection of MCF-7 cells in complex samples showed that the SNCE biosensors have great potential for patient sample detection.

Graphical Abstract

Keywords

Circulating tumor cells; Single-nanoparticle collision electrochemistry; Immunomagnetic separation; Liposome; Platinum nanoparticles

DOI Link

https://doi.org/10.61558/2993-074X.3483

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Publication Date

2024-10-28

Online Available Date

2024-06-09

Revised Date

2024-06-05

Received Date

2024-04-29

Recommended Citation

Fu-Xing Qin, Ming-Ke Li, Hui-Long Zhou, Wei Wen, Xiu-Hua Zhang, Sheng-Fu Wang, Zhen Wu. Platinum Nanoparticle-Based Collision Electrochemistry for Rapid Detection of Breast Cancer MCF-7 Cells[J]. Journal of Electrochemistry, 2024 , 30(10): 2414004.
DOI: 10.61558/2993-074X.3483
Available at: https://jelectrochem.xmu.edu.cn/journal/vol30/iss10/2