Precision Delivery Using Nanopipette for Single-Cell Studies

Authors

He Zhang, Department of Chemistry, The University of Texas at Austin Austin, TX 78712, USA
Md Maksudur Rahman, Department of Chemistry, The University of Texas at Austin Austin, TX 78712, USA
Yang Tao, Department of Chemistry, The University of Texas at Austin Austin, TX 78712, USA
Joseph W. Sampson, Department of Chemistry, The University of Texas at Austin Austin, TX 78712, USA
Hang Ren, Department of Chemistry, The University of Texas at Austin Austin, TX 78712, USA; Center for Electrochemistry, The University of Texas at Austin, Austin, TX 78712, USA; Texas Materials Institute, The University of Texas at Austin, Austin, TX 78712, USA

Document Type

Review

Corresponding Author(s)

Hang Ren(hren@utexas.edu)

Abstract

Nanopipette based scanning probe technique is a versatile tool in non-contact imaging in biology. In addition to the topographic imaging, its capability of localized delivery of bio-active molecules is emerging. In this mini review, we introduced the applications of nanopipette in single-cell research with a focus on localized delivery. The working principles of three delivery modes were summarized and compared: resistive pulse, pressure-driven flow, and electroosmotic flow-driven delivery. Their applications in single-cell research are reviewed. The current technical challenges in SICM-based delivery and their growing influence in medicine and pharmacology research are also discussed.

Graphical Abstract

Keywords

Scanning Ion Conductance Microscopy, Nanopipette, Single Cell Image, Local Delivery

DOI

10.61558/2993-074X.3489

Online Date

8-21-2024

Recommended Citation

He Zhang, Md Maksudur Rahman, Yang Tao, Joseph W Sampson, and Hang Ren. Precision Delivery Using Nanopipette for Single-Cell Studies[J]. Journal of Electrochemistry, doi: 10.61558/2993-074X.3489.