Precision Delivery Using Nanopipette for Single-Cell Studies

He Zhang, Department of Chemistry, The University of Texas at Austin, Austin, TX 78712, USA
Maksudur Rahman, Department of Chemistry, The University of Texas at Austin, Austin, TX 78712, USA
Yang Tao, Department of Chemistry, The University of Texas at Austin, Austin, TX 78712, USA
Joseph W Sampson, Department of Chemistry, The University of Texas at Austin, Austin, TX 78712, USA
Hang Ren, a Department of Chemistry, The University of Texas at Austin, Austin, TX 78712, USA; b Center for Electrochemistry, The University of Texas at Austin, Austin, TX 78712, USA; c Texas Materials Institute, The University of Texas at Austin, Austin, TX 78712, USA Follow

Corresponding Author

Hang Ren (hren@utexas.edu)

Abstract

Nanopipette based scanning probe technique is a versatile tool in non-contact imaging in biology. In addition to the topographic imaging, its capability of localized delivery of bio-active molecules is emerging. In this mini review, we introduce the applications of nanopipette in single-cell researches with a focus on localized delivery. The working principles of three delivery modes including resistive pulse, pressure-driven flow, and electroosmotic flow-driven delivery are summarized and compared. Their applications in single-cell researches are reviewed. The current technical challenges in scanning ion conductance microscopy-based delivery, and their growing influence in medicine and pharmacologic researches are also discussed.

Graphical Abstract

Keywords

Scanning ion conductance microscopy, Nanopipette, Single cell image, Local delivery

DOI Link

https://doi.org/10.61558/2993-074X.3488

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Publication Date

2024-11-28

Online Available Date

2024-08-21

Revised Date

2024-08-07

Received Date

2024-04-29

Recommended Citation

He Zhang, Maksudur Rahman, Yang Tao, Joseph W Sampson, Hang Ren. Precision Delivery Using Nanopipette for Single-Cell Studies[J]. Journal of Electrochemistry, 2024 , 30(11): 2414002.
DOI: 10.61558/2993-074X.3488
Available at: https://jelectrochem.xmu.edu.cn/journal/vol30/iss11/3

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