Abstract
Glucose oxidase (GOx)-gold nanoparticles (AuNPs) composite was synthesized by the one-pot enzyme reaction in the mixture solution of NaAuCl4, GOx and glucose, and a Nafion/GOx-AuNPs/glassy carbon electrode was fabricated by the cast-coating method to examine the direct electrochemistry of GOx and the biosensing performance on this electrode. Such a GOx-AuNPs composite showed good direct-electrochemistry activity and bioactivity of GOx, probably because the enzyme-mediated AuNPs are close to the redox active centers of GOx. This enzyme electrode exhibited a linear amperometric response to glucose concentration (0.54 mmol·L-1) at -0.4 V (vs. SCE), and a detection limit of 0.2 mol·L-1 was obtained.
Graphical Abstract
Keywords
Glucose oxidase, enzymatic synthesis, glucose oxidase-Au nanoparticles composite, direct electrochemistry, biosensing
Publication Date
2014-12-28
Online Available Date
2014-07-26
Revised Date
2014-07-21
Received Date
2014-05-27
Recommended Citation
Fang HE, Xiao-li QIN, Ying-chun FU, Chao CHEN, Qing-ji XIE, Shou-zhuo YAO.
Direct Electrochemistry and Glucose Biosensing of Glucose Oxidase-Gold Nanoparticles Composite Synthesized by Enzyme Method[J]. Journal of Electrochemistry,
2014
,
20(6): 515-520.
DOI: 10.13208/j.electrochem.140434
Available at:
https://jelectrochem.xmu.edu.cn/journal/vol20/iss6/3
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