Direct Electrochemistry and Glucose Biosensing of Glucose Oxidase-Gold Nanoparticles Composite Synthesized by Enzyme Method

Authors

Fang HE, Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, China;
Xiao-li QIN, Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, China;
Ying-chun FU, College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310029, China;
Chao CHEN, Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, China;
Qing-ji XIE, Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, China;Follow
Shou-zhuo YAO, Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, China;

Corresponding Author

Qing-ji XIE(xieqj@hunnu.edu.cn)

Abstract

Glucose oxidase (GOx)-gold nanoparticles (AuNPs) composite was synthesized by the one-pot enzyme reaction in the mixture solution of NaAuCl₄, GOx and glucose, and a Nafion/GOx-AuNPs/glassy carbon electrode was fabricated by the cast-coating method to examine the direct electrochemistry of GOx and the biosensing performance on this electrode. Such a GOx-AuNPs composite showed good direct-electrochemistry activity and bioactivity of GOx, probably because the enzyme-mediated AuNPs are close to the redox active centers of GOx. This enzyme electrode exhibited a linear amperometric response to glucose concentration (0.54 mmol·L^-1) at -0.4 V (vs. SCE), and a detection limit of 0.2 mol·L^-1 was obtained.

Graphical Abstract

Keywords

Glucose oxidase, enzymatic synthesis, glucose oxidase-Au nanoparticles composite, direct electrochemistry, biosensing

DOI Link

https://doi.org/10.13208/j.electrochem.140434

Publication Date

2014-12-28

Online Available Date

2014-07-26

Revised Date

2014-07-21

Received Date

2014-05-27

Recommended Citation

Fang HE, Xiao-li QIN, Ying-chun FU, Chao CHEN, Qing-ji XIE, Shou-zhuo YAO. Direct Electrochemistry and Glucose Biosensing of Glucose Oxidase-Gold Nanoparticles Composite Synthesized by Enzyme Method[J]. Journal of Electrochemistry, 2014 , 20(6): 515-520.
DOI: 10.13208/j.electrochem.140434
Available at: https://jelectrochem.xmu.edu.cn/journal/vol20/iss6/3