Development of dehydrogenase-based bioanode using poly(phenosafranin)-functionalized SWCNT nanocomposites and its application to ethanol biosensor

Authors

S. Saleh Farhana, Department of Electronic Chemistry, Interdisciplinary Graduate School of Science and Engineering, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama 226-8502, Japan
Okajima Takeyoshi, Department of Electronic Chemistry, Interdisciplinary Graduate School of Science and Engineering, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama 226-8502, Japan
Mao Lanqun, National Laboratory for Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences(CAS),China
Takeo Ohsaka, Department of Electronic Chemistry, Interdisciplinary Graduate School of Science and Engineering, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama 226-8502, Japan

Corresponding Author

Takeo Ohsaka and

Abstract

A New type of dehydrogenase-based amperometric ethanol biosensor was constructed using alcohol dehydrogenase (ADH) which was immobilized on the edge-plane pyrolytic graphite (EPPG) electrode modified with poly(phenosafranin)-functionalized single-walled carbon nanotube (PPS-SWCNT). The PPS-SWCNT modified EPPG electrode was prepared by electropolymerization of phenosafranin on the EPPG electrode which was previously coated with SWCNT. The performance of the ADH/PPS-SWCNT/EPPG electrode was evaluated using cyclic voltammetry and amperometry in the presence of ethanol. The fabricated ethanol biosensor provided a reasonable sensitivity of 2.0 μA cm–2 mM–1 and a low detection limit (36 μM) for the electrocatalytic oxidation of ethanol with a linear concentration dependence upto ~ 1.0 mM at a detection potential of 0.2 V.

Graphical Abstract

Keywords

Phenosafranin, electropolymerization, NADH, SWCNT, electrocatalysis

DOI Link

https://doi.org/10.61558/2993-074X.2093

Publication Date

2011-08-28

Online Available Date

2011-07-20

Revised Date

2011-07-11

Received Date

2011-06-07

Recommended Citation

S. Saleh Farhana, Okajima Takeyoshi, Mao Lanqun, Takeo Ohsaka. Development of dehydrogenase-based bioanode using poly(phenosafranin)-functionalized SWCNT nanocomposites and its application to ethanol biosensor[J]. Journal of Electrochemistry, 2011 , 17(3): 263-270.
DOI: 10.61558/2993-074X.2093
Available at: https://jelectrochem.xmu.edu.cn/journal/vol17/iss3/2