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Abstract

A novel carbon paste electrode(CPE) modified with MnO2 and glucose oxidase(GOx),is reported.Different factors,such as the effect of supporting electrolyte,pH of solutions,various paste glues,the amount of MnO2 and temperature,which may influence the determination of H2O2 and glucose were tested to optimize.Based on above,potentiometric determination of glucose was achieved in neutral solution and at 35 ℃,the electrode gave a response of 36.12~34.62 mV/pGlucose between 2.0×10-5mol·L-1 and 5.2×10-3 mol·L-1 with a detection limit of 3.3×10-6 mol·L-1.Moreover,this kind of the electrode had good stability and reproducibility.Most normal anion,cation,isotol,glycerin and amide will not interfere the determination of glucose.

Keywords

MnO2-GOx, carbon paste electrode, glucose, potentiometric response

Publication Date

2008-02-28

Online Available Date

2008-02-28

Revised Date

2008-02-28

Received Date

2008-02-28

References

[1]Janata J,Josowicz M,Vanysek P,et al.Chemical sen-sors[J].Anal Chem,1998,70:179R.
[2]Wang J.Electroanalysis and biosensors[J].AnalChem,1999,71:328R.
[3]Zhu L,Li Y Z,Ci Y X.Microbial based sensor for themeasurement of glucose in sermn and urine[J].ActaChimica Sinica,2002,04:23.
[4]Henry C.Getting under the skin:implantable glucosesensors[J].Anal Chem,1998,70:594A.
[5]Kirk J K,Rheney C C.Important features of blood glu-cose meters[J].J Am Pharm Assoc,1998,38:210.
[6]Clark LC,Lyons Jr C.Electrode systems for continuousmonitoring in cardiovasculasurgery[J].Ann NY AcadSci,1962,102:29.
[7]Soldatkin A P,El'skaya A V,Shul'ga A A,et al.Glu-cose sensitive conductometric biosensor with additionalNafion membrane:reduction on influence of buffer ca-pacity on the sensor response and extension of its dy-namic range[J].Anal Chim Acta,1993,283:695.
[8]Kharitonov A B,Zayats M,Lichtenstein A,et al,En-zyme monolayer-functionalized field-effect transistors forbiosensor applications[J].Sens Actuators B.2000,70:222.
[9]Yin L T,Chou J C,Chung W Y,et al.Glucose EN-FET doped with MnO2powder[J].Sens Actuators B,2001,76:187.
[10]Shul'ga A A,Koudelka-Hep M,Rooij de,et al.Glu-cose-sensitive enzyme field effect transistor using potas-sium ferricyanide as an oxidizing substrate[J].AnalChem,1994,66:205.
[11]Zhang H(张宏),Jin B K(金葆康),Jin X Y(金晓燕).The study of electrochemical behavior of dopa-mine at nano-gold modified carbon fiber electrode[J].Chinese Chemical Letters(in Chinese),2003,07:49.
[12]Silvia A Miscoria,Gustavo D Barrera,Gustavo A Ri-vas et al,Analytical performance of a glucose biosensorprepared by immobilization of glucose oxidase anddif-ferentmetals into a carbonpasteelectrode[J].Electroa-nalysis,2002,14(14):981.
[13]Wang J,Naser N,Angnes L,et al.Metal-dispersedcarbon paste electrodes[J].Anal Chem,1992,64:1285.
[14]Schachl K,Turkusˇic E,Komersova A,et al.Thethree-electrode configurations are planer Beckman mod-el[J].Collect Czech Chem Comm,2002,67:302.
[15]Karayakin A A,Kotel'nikova E A,Lukachova L V,etal.Optimal environment for glucose oxidase in perfluo-rosulfonated ionomer membranes:Improvement of first-generation biosensors[J].J Anal Chem,2002,74:1597.
[16]Zheng X W,Guo Z H.Flow-injection chemilumines-cence determination of quinine using on-line electro-generated cobalt(Ⅲ)as oxidant[J].Talanta,2000,50:1157.
[17]Luo Xi-Liang,Xu Jing-Juan,Zhao Wei,et al.A no-vel glucose ENFET based on the special reactivity ofMnO2nanoparticles[J].Biosensors and Bioelectron-ics,2004,19:1295.
[18]Luo Xi-Liang,Xu Jing-Juan,Zhao Wei,et al.Glu-cose biosensor based on ENFETdoped with SiO2nano-particles[J].Sensors and Actuators B,2004,97:249.

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