Detection of Acute Promyelocytic Leukemia PML/RARα Fusion Gene Using Graphene Modified Glassy Carbon Electrode

Authors

Zhi-xian LIAN, 1. Department of Pharmaceutical Analysis of the Fujian Meidical University, Fuzhou 350004,China; ;
Guang-xian ZHONG, 1. Department of Pharmaceutical Analysis of the Fujian Meidical University, Fuzhou 350004,China; ;
Kun WANG, 1. Department of Pharmaceutical Analysis of the Fujian Meidical University, Fuzhou 350004,China; ; 3.Department of Pharmacy, the First Hospital of Xiamen Affiliated to the Xiamen University, Xiamen 361003, Fujian, China;
Ai-lin LIU, 1. Department of Pharmaceutical Analysis of the Fujian Meidical University, Fuzhou 350004,China; ; 2. Fujian Institute of Hematology, the Affiliated Union Hospital of Fujian Medical University, Fuzhou 350001, China; ;Follow
Xin-hua LIN, 1. Department of Pharmaceutical Analysis of the Fujian Meidical University, Fuzhou 350004,China; ;Follow
Yuan-zhong CHEN, 2. Fujian Institute of Hematology, the Affiliated Union Hospital of Fujian Medical University, Fuzhou 350001, China; ;

Corresponding Author

Ai-lin LIU(ailinliu@mail.fjmu.edu.cn);
Xin-hua LIN(xinhua63@163.com)

Abstract

A graphene modified glassy carbon electrode (GCE) was prepared by electrochemical reduction of graphite oxide fixed on the surface of GCE. Then, the amino-group modified probe of acute promyelocytic leukemia (APL) PML/RARα fusion gene fragment was immobilized onto surface of the graphene modified GCE by coupling activating agent. With methylene blue (MB) as a novel electrochemical indicator, artificial APL PML/RARα fusion gene fragment was measured by differential pulse voltammetry (DPV). The result indicated graphene nanomaterial showed good sensitization on the signal of MB. The relationship between the reduction peak current of MB and the concentration of complementary strand was linear in the range of 5×10-10~2.5×10-9 mol/L. The detection limit had been estimated as 8×10-11 mol/L. The method is simple, good specificity and high sensitivity. This approach is promising to the detection of APL in the real sample for medical diagnostics.

Graphical Abstract

Keywords

Graphene, electrochemical DNA biosensor, PML/RAR&alpha, fusion gene

DOI Link

https://doi.org/10.61558/2993-074X.2089

Publication Date

2011-05-28

Online Available Date

2011-05-06

Revised Date

2011-02-25

Received Date

2011-01-14

Recommended Citation

Zhi-xian LIAN, Guang-xian ZHONG, Kun WANG, Ai-lin LIU, Xin-hua LIN, Yuan-zhong CHEN. Detection of Acute Promyelocytic Leukemia PML/RARα Fusion Gene Using Graphene Modified Glassy Carbon Electrode[J]. Journal of Electrochemistry, 2011 , 17(2): 195-198.
DOI: 10.61558/2993-074X.2089
Available at: https://jelectrochem.xmu.edu.cn/journal/vol17/iss2/9

References

[1] GeimA K，Novoselov K S．The rise of graphene[J]. Nature Mater，2007, 6(3): 1 183 - 191.


[2] Shan C S, Yang H F, Han D X, et al. Water-Soluble Graphene Covalently Functionalized by Biocompatible Poly-L-lysine [J]. Langmuir, 2009, 25(20) :12030-12033.


[3] Fu C L, Yang W S, Chen X, et al．Direct electrochemistry of glucose oxidase on a graphite nanosheet-Nafion composite film modified electrode[J]. Electrochem Commun, 2009, 11(5)：997-1000.


[4] Zhang J Y(张建瑜)，Tang J M (唐加明). Clinic study about 21 cases of acute promyelocytic leukemia [J]. International Medicine & Health Guidance News (国际医药卫生导报), 2007, 13(12): 28-31.


[5] Yoo S J, Seo E J, Lee J H, et al. A complex, four-way variant t(15; 17) in acute promyelocytic leukemia[J]. Cancer Genet Cytogenet, 2006, 167 (2): 168-171.


[6] Han J Y, Kim K E, Kim K H, et al. Identification of PML-RARA rearrangementbyRT-PCR and sequencing in an acute promyelocytic leukemiawithout t(15; 17)on G-banding and FISH[ J]. Leukemia Research, 2007, 31(2): 239-243.


[7] Wei N, Chen J H, Zhang J, et al. An electrochemical biosensor for detection of PML/RARA fusion gene using capture probe covalently immobilized onto poly-calcon carboxylic acid modified glassy carbon electrode [J]. Talanta, 2009, 78 (4-5): 1227-1234.


[8] Guo H L, Wang X F, Qian Q Y, et al. A Green approach to the synthesis of graphene nanosheets[J]. ACS Nano, 2009, 3 (9) : 2653€“2659.


[9] Hummers W S, Offeman R E. Preparation of graphite oxide [J]. J Am Chem Soc, 1958, 80(6):1339-1339．


[10] Nethravathi C, Viswanath B, Shivakumara C, et al. The production of semectite clay/graphene composites through delamination and co-stacking[J]. Carbon, 2008, 46(13): 1773-1781.


[11] Erdem, A, Kerman K, Meric B, et al. Methylene blue as a novel electrochemical hybridization indicator[J]. Electroanalysis, 2001, 13(3):219-223.


[12] Lin X H, Wu P, Chen W, et al. Electrochemical DNA biosensor for the detection of short DNA species of chronic myelogenous leukemia by using methylene blue [J]. Talanta, 2007, 72 (3): 468-471.