Detection of PML/RARα Fusion Gene Using Gold Nanopaticles Modified Electrode by Chronocoulometry

Authors

Li-Man WANG
Li-Qing LIN
Shao-Huang WENG
Xin-Hua LIN
Yuan-Zhong CHEN

Corresponding Author

Xin-Hua LIN(xinhua63@163.com)

Abstract

potential. The sulfydryl modified probe was immobilized onto surface of gold nanoparticles via Au-S covalent bond and hybridized with complementary target DNA sequences, then the DNA sensor was fabricated for the detection of PML/RARα fusion gene by chronocoulometry in acute promyelocytic leukemia (APL). Scanning electron microscopy (SEM) and electrochemical impedance spectroscopy (EIS) were used to characterize the surface morphology of gold nanoparticles and the constructing process of sensor. With hexaammineruthenium(Ⅲ) chloride (RuHex) as a novel electrochemical indicator, the chronocoulometric DNA biosensor was employed to monitor artificial APL PML/RARα fusion gene fragment．Experimental results showed that the gold nanoparticles amplified the detection signal of RuHex. The linear relationship between the charge difference in the RuHex after and before hybridization and logarithmic concentrations (log C) of target sequence was observed in the concentration range of 1.0×10-13 ~ 1.0×10-9 mol?L-1, and the detection limit had been estimated to be 3.7×10-14 mol?L-1 (S/N=3). This approach is simple, has good specificity, and is promising to apply for the detection of APL in the real sample for medical diagnostics.

Graphical Abstract

Keywords

gold nanoparticles, chronocoulometric, RuHex, PML/RAR&alpha, fusion gene

DOI Link

https://doi.org/10.61558/2993-074X.2917

Publication Date

2012-06-28

Online Available Date

2012-02-19

Revised Date

2012-02-09

Received Date

2012-01-17

Recommended Citation

Li-Man WANG, Li-Qing LIN, Shao-Huang WENG, Xin-Hua LIN, Yuan-Zhong CHEN. Detection of PML/RARα Fusion Gene Using Gold Nanopaticles Modified Electrode by Chronocoulometry[J]. Journal of Electrochemistry, 2012 , 18(3): Article 15.
DOI: 10.61558/2993-074X.2917
Available at: https://jelectrochem.xmu.edu.cn/journal/vol18/iss3/15

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