Abstract
In this article, we developed a novel DNA electrochemical sensor for obtaining a current-time curve based on DNA polymerase I. The capture probes were fixed on the Au electrode by Au interacting with -SH, and then hybridized with the K-ras mutant target DNA as far as one base before the mutation site. After hybridizing the K-ras mutant or wild target DNA, the resulting assembly reacted with the dUTP-biotin in the presence of DNA polymerase I. Then avidin-HRP combined the dUTP-biotin on the electrode. The proposed electrode was applied for detection in TMB solution by electrochemical technology. The experimental results showed that the detection current of the proposed DNA electrochemical sensor is proportional to the logarithmic K-ras mutant DNA concentration (1.0×10-15 to 1.0×10-10 mol·L-1). It had high sensitivity and good selectivity.
Graphical Abstract
Keywords
DNA polymerase I, K-ras point mutant gene, DNA electrochemical sensor, current-time curve
Publication Date
2015-02-28
Online Available Date
2014-10-08
Revised Date
2014-09-26
Received Date
2014-07-25
Recommended Citation
Li-qing LIN, Cheng-fei ZHAO, Zhou-qian JIANG, Shao-huang WENG, Xiao-lan XIE, Xin-hua Lin.
Novel DNA Electrochemical Biosensor for K-Ras Point Mutation Detection Based on DNA Polymerase I[J]. Journal of Electrochemistry,
2015
,
21(1): 72-77.
DOI: 10.13208/j.electrochem.140725
Available at:
https://jelectrochem.xmu.edu.cn/journal/vol21/iss1/11
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