An Aptamer-Based Microelectrode with Tunable Linear Range for Monitoring of K⁺ in the Living Mouse Brain

Authors

Yuan-Dong Liu, Shanghai Key Laboratory of Green Chemistry and Chemical Processes, Department of Chemistry, School of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200241, China
Jia-Run Li, Shanghai Key Laboratory of Green Chemistry and Chemical Processes, Department of Chemistry, School of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200241, China
Li-Min Zhang, Shanghai Key Laboratory of Green Chemistry and Chemical Processes, Department of Chemistry, School of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200241, ChinaFollow
Yang TianFollow

Corresponding Author

Li-Min Zhang(lmzhang@chem.ecnu.edu.cn);
Yang Tian (ytian@chem.ecnu.edu.cn)

Abstract

Potassium ion (K⁺) is widely involved in several physiopathological processes, and its abnormal changes are closely related to the occurrence of brain diseases of cerebral ischemia. In vivo acquirement of K⁺ variation is significant to understand the roles of K⁺ playing in brain functions. A microelectrode based on single-stranded DNA aptamers was developed for highly selective detection of K⁺ in brain, in which the aptamer probes were designed to contain an aptamer part for specific recognition of K⁺, an alkynyl group used for stable confinement of aptamer probe on the gold surface, and an electrochemical redox active ferrocene group to generate current response signal. The response range of the microelectrodes could be rationally tuned by varying the chain length of the aptamer probe. The optimized electrode, LAC, displayed high selectivity for in vivo detection of K⁺, and suitable linear range from 10 μmol·L^–1–10 mmol·L^–1, which could fulfill the requirement of K⁺ detection in brain. Eventually, the microelectrodes were successfully applied for the detection of K⁺ in the living mouse brains followed by hypoxic.

Graphical Abstract

Keywords

Aptamer; Functional microelectrode; Potassium ion; Brain

DOI Link

https://doi.org/10.13208/j.electrochem.2218004

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Publication Date

2023-06-28

Online Available Date

2023-05-20

Revised Date

2023-05-12

Received Date

2022-11-14

Recommended Citation

Yuan-Dong Liu, Jia-Run Li, Li-Min Zhang, Yang Tian. An Aptamer-Based Microelectrode with Tunable Linear Range for Monitoring of K⁺ in the Living Mouse Brain[J]. Journal of Electrochemistry, 2023 , 29(6): 2218004.
DOI: 10.13208/j.electrochem.2218004
Available at: https://jelectrochem.xmu.edu.cn/journal/vol29/iss6/13