Molecular Inclusion of Cyclodextrin Polymer and Its Application to Enzyme Electrodes

Authors

Huihuang Wu
Baozhang WU

Abstract

The inclusion complexation of α-cyclodextin prepolymer host with benzoquinone guest was studied by voltametry. The spectroscopic measurements show that the polycondensation of CD with glutaric dialdehyde forms the polymer (PCD) with pending carbonyl, the latter brings about covalent immobilisation of glucose oxidase (GOD) to polymer chains. Owing to the inclusion complexation of CD, the benzoquinone(BQ) as mediator of GOD can be collected in the enzymecontaining PCD membrane, resulting in high concentration of BQ in the membrane while decreasing its amount in solution. TTF was tested to prepare the immobilised enzyme together with mediator in PCD. The enzyme loading in PCD membrane and the membrane thickness can be controlled easily, which will facilate to fundmental reshearch of enzyme electrodes.

DOI Link

https://doi.org/10.61558/2993-074X.2695

Publication Date

1998-05-28

Online Available Date

1998-05-28

Revised Date

1998-05-28

Received Date

1998-05-28

Recommended Citation

Huihuang Wu, Baozhang WU. Molecular Inclusion of Cyclodextrin Polymer and Its Application to Enzyme Electrodes[J]. Journal of Electrochemistry, 1998 , 4(2): Article 15.
DOI: 10.61558/2993-074X.2695
Available at: https://jelectrochem.xmu.edu.cn/journal/vol4/iss2/15

References

GrundigB，KrabischC．Electronmediatormodofiedelectrodesforthedeterminationofglucoseinfermentationmedia．Anal．Chim．Acta，1989，222：75～812SakuraS，BuckRP．AmperometricproceseswithGODembeddedintheelectrode．Bioelectrochem．Bioenerg．，1992，28：387～4003FouldsNC，LoweCR．Immobilizationofglucoseoxidaseinferocenemodifiedpyrolepolymers．Anal．Chem．，1988，60：2473～24784吴辉煌，郑智敏，周绍民．生物功能电极（I），化学学报，1991，49：689～6935TabakovicR，TabakovicI，DavidovicA．Glucoseoxidaseimmobilizedinapolycationicpolymercontainingferroceneonaplatnumelectrode．J．Electroanal．Chem．，1992，332：297～3016WilnerI，KasherR，ZahavyE，LapidotN．MediatedelectrontransferinglutathionereductaseorganizedinselfasembledmonolayersonAuelectrode．J．Am．Chem．Soc．，1992，114：10965～109667HelerA．Electricalwiringofredoxenzymes．Ac．Chem．Res．，1990，23，128～1348KutnerW，StockW，DoblhoferK．Preparationandpropertiesofinsolublefilmsofcyclodextrincondensationpolymers．J．Incl．Phenom．，1992，13：257～2659SzejtliJ．CyclodextrinsandtheirInclusionComplexes．Budapest：AkademiaiKiado，198210YamadaK，KohmotoS，IidaH．Theinclusionefectsofcyclodextrinsonphotolysisofpbenzoquenoneinaqueoussoltuion．Bul．Chem．Soc，Jpn．，1976，49：1171～117211MortonRA．BiochemicalSpectroscopy．NewYork：Wiley，1975：20712BourdilonC，HervagaultC，ThomasD．Increaseinoperationalstabilityofimmobilizedglucoseoxidasebytheofartifitialcosubstrates．Biotech．Bioeng．，1985，27：1619～162213MarchesieloM，GeniesEM．Glucosesensor：polypyroleglucoseoxidaseelectrodeinthepresenceofpbenzoquenone．Electrochim．Acta，1992，37：1987～1992