Abstract
It is shown that calf thymus DNA(CT DNA) can be adsorbed at highly ordered pyrolytic graphite(HOPG) electrode and detected by differential pulse voltammetry(DPV) and electrochemical atomic force microscopy(ECAFM). It is found that controlled potential pre-polarization influences largely the adsorption of dsDNA while it has little influence on ssDNA. It is demonstrated that ECAFM is useful to exploit adsorption of DNA at HOPGE. The pattern of adsorption of DNA at HOPG electrode that fits best with the experimental data is proposed. The results aslo shows that UV/Vis reflection spectroscopy, which has not been used in studying electrochemical behavior of DNA, is more sensitive than voltammetry in the investigation of DNA adsorption.
Keywords
DNA, HOPG, Pre_polarization, Oxidation, ECAFM, UV/Vis reflection spectroscopy
Publication Date
1999-05-28
Online Available Date
1999-05-28
Revised Date
1999-05-28
Received Date
1999-05-28
Recommended Citation
Jianzhang Zhou, Lingling Wu, Jin Luo, Zhonghua Lin.
Oxidation and Adsorption of Deoxyribonucleic Acid at Highly Ordered Pyrolytic Graphite and
Glass Carbon Electrode[J]. Journal of Electrochemistry,
1999
,
5(2): Article 11.
DOI: 10.61558/2993-074X.3174
Available at:
https://jelectrochem.xmu.edu.cn/journal/vol5/iss2/11
References
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