Abstract
In this paper,the electrochemical response characteristics of enzyme and immuno electrodes were studied by cyclic voltammetry under different conditions. Our goals were to determine the best modified way of bioelectrode,which was the base of biosensor's application. 30U GOD and 0.8 mg/mL SEC1 antibody were suitable to bioelectrode preparation and detection. The microscopical analysis was finished with scan microscopy.
Publication Date
2002-02-28
Online Available Date
2002-02-28
Revised Date
2002-02-28
Received Date
2002-02-28
Recommended Citation
Sa-ying DONG, Hong-ren WANG, Yu-zhu GAO, Guang-zhang CHEN, Ming-xiang LV, Bo-yi TIAN.
The Determination of the Best Modified Way and Microscopical Analysis for Enzyme/immunoelectrodes[J]. Journal of Electrochemistry,
2002
,
8(1): Article 15.
DOI: 10.61558/2993-074X.3281
Available at:
https://jelectrochem.xmu.edu.cn/journal/vol8/iss1/15
References
[1] LeiZuorong.StaphylococcusAureusToxinandItsDisease[M ].Beijing:ChineseScienceandTechnologyPress(inChinese),1992.
[2] GuoShengqing,SongFengqing,GuoJun,etal.Newadvanceondetectingbiologicaltoxin[J].JournalofHy gieneResearch(inChinese),1998,27,supp1:110.
[3] YuH .Useofanimmunomagneticseparation_fluorescentimmunoassay(IMS_FIA )forrapidandhighthroughputanalysisofenvironmentalwatersamples[J].Anal.Chim.Acta,1998,376:77.
[4] YuH ,AhmedH ,VastaGR .Developmnetofamagneticmicroplatechemisluorimmunoassayforrapiddectionofbacteriaandtoxininbollod[J].AnalBiochem,1998,261:1.
[5] SanchezFG ,DiazAN ,DiazAFG ,etal.Quantificationof2,4,5_trichlorophenoxyaceticacidbyfluores cenceenzyme_linkedimmunosorbentassaywithsecondaryantibody[J].Anal.Chim.Acta,1999,378:219.
[6] WangYing,DongShaojun,etal.Cryoimmobilizedenzymeforbiosensorconstruction[J].SensorsandActua torsB ,1996,31:193.
[7] WrightDS ,HalsallHB ,HeinemanWR .Digoxinhomogeneousenzymeimmunoassayusinghigh_perfor manceliquidchromatographiccolumnswitchingwithamperometicdetection[J].Anal.Chem.,1986,58:2995.
[8] DongSaying,LuMingxiang,HuangZhencai.InSituFTIRreflectionspectroscopyofHCGenzymeimmuno electrode[J].ChineseJournalofBiomedicalEngineering(inChinese),1995,14(2):107.
[9] DongSaying,LuMingxiang,LuoGuoan.ElectrochemicalcharacteristicsofHCGenzymeimmunoelectrode[J].JournalofAnalyticalScience(inChinese),2000,16(3):234.
[10] DongSaying,LiuXinmiao,etal,Determinationofkineticparametersofenzyme_immunoelectrodeforSEC1Assay[J].Electrochemistry(inChinese),2000,6(2):175.
[11] RobinsonGA ,ColeVM ,RattleSJ,etal.Bioelectrochemicalimmunoassayforhumanchorionicgo nadotrophininserumusinganelectrode_immobilisedcaptureantibody[J].Biosensors,1986,2:45.
[12] RobinsonGA ,ColeVM ,ForrestGC .Ahomogeneouselectrode_basedbioelectrochemicalimmunoassayforhumanchorionicgonadotrophin[J].Biosensors,1987,88(3):147
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