A new approach to construct an ampermetric biosensor was described.The 4,4-bis(methanethiol)biphenyl(MTP)is adsorbed on to a bare gold electrode to make self-assembled monolayers(MTP/Au SAMs).Then electrodeposited 2mg/mL HAuCl4 at-200 mV,NG/MTP/Au electrode was prepared.When a differential pulse voltammetric(DPV)technique was used,the peak separation between DA and AA is 155 mV.Hence,the selective determination of dopamine(DA)and ascorbate(AA)in the binary mixture system can be performed.Under the chosen conditions,the peak currents are correspondent linearly to the concentrations of DA and AA in the range of 5.0×10-7~1×10-4 mol·L-1 and 3.5×10-6~1.0×10-3mol·L-1,respectively.The correlation coefficients were 0.998.The detection limit were 1.5×10-7 mol·L-1 and 1.2×10-6 mol·L-1for DA and AA.


dopamine, ascorbate, gold nanpoarticle, MTP, DPV

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[1]Stamford J A,Justice J B Jr.Probing brain chemistry with electroanalytical techniques-voltammetry comes of age[J].Anal Chem,1996,68:359A-363A.
[2]Boulton A,Baker G,Adams R N.Carbon electrode sur-face chemistry:optimization of bioanalytical perform-ance[M].In:Voltammetric Methods in Brain Systems,Edited by Boulton A A,Baker G B,Adams R N,To-towa:Humana Press,NJ,1995.1-26.
[3]Zhang L,Jiang X.Electrochemical behavior of querce-tin:Experimental and theoretical studies[J].Electroa-nalytical Chemistry.2005,583:292299.
[4]Jahan-Bakhsh Raoofr,Reza Ojani,Sahar Rashid-Nadi-mi.Voltammetric determination of ascorbic acid and do-pamine in the same sample at the surface of a carbon paste electrode modified with polypyffole/feffocyanide.[J].Electrochimica Acta,2005,50:46944698.
[5]Zhao Y,Gao Y,Zhan D,et al.Selective detection of dopamine in the presence of ascorbic acid and uric acid by a carbon nanotubes-ionic[J].Talanta,2005,66:51-57.
[6]Raj C R,Tokuda K,Ohsaka T.Electroanalytical appli-cations of cationic self-assembled monolayers:square-wave voltammetric determination of dopamine and ascor-bate[J].Bioelectrochemistry.2001,53:183-191.
[7]Zhou Y,Hu S,Cao Z,et al.Capacitive immunosensor for the determination of Schistosoma japonicum antigen[J].Analytical Letters,2002,35:1919-1930.
[8]Raj C R,Okajima T,Ohsaka T.Gold nanoparticle ar-rys for the voltammetric sensing of dopamine[J].Elec-troanalytical Chemistry.2003,543:127-133.
[9]Zhang P,Wu F,Zhao G,et al.Selective response of dopamine in the presence of ascorbic acid at multi-walled carbon nanotube modified gold electrode[J].Bioelectrochemistry.2005,67:109-114.
[10]Jin B,Ding S,Kametani K,et al.The preparation and electrochemical behavior of density-controlled gold nano-particle sself-assembled interface[J].Chemistry Letters,2005,34:302-303
[11]Liu S,Li X.The influence of gold nanoparticle modi-fied electrode on the structure of mercaptopropionic acid self-assembly monolayer[J].Electrochimica Ac-ta,2005,51:427-431.
[12]Jia L(贾莉),Lei Q(雷秋芬),Zhang X(张修华),et al.Determination of dopamine on o-aminobenzeneth-iol self-assembled monolayers modified gold electrode[J].Application Chemistry(应用化学),2005,22:172-175.
[13]Raj C R,Okajima T,Ohsaka T.Gold nanoparticle ar-rys for the voltammetric sensing of dopamine[J].Elec-troanalytical Chemistry,2003,543:127-133.
[14]Zhang H(张宏),Gui X Q(桂学琴),Jin B K(金葆康).Nanogold-modified glassy carbon electrode for selective detection of dopamine in the presence of as-corbic acid[J].Journal of Analytical Science(分析科学学报),2002,18:194-198.



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