Hemoglobin(Hb) was immobilized onto the surface of glassy carbon electrode with the single-wall carbon nanotubes and surfactant didodecyldimethylamonium bromide(DDAB) modified previously and its electrochemical behaviors have been investigated by cyclic voltammetry.A pair of well-defined redox peaks has been obtained.Cyclic voltammetry and electrochemical impedance spectroscopy(EIS) were also used to study on the effect of different concentration of SWCNT in DDAB.The result shows that the 1 mg/mL is the best choice.The dependence of E0' on solution pH with the slope of 39 mV/pH,indicated that redox of the Hb is a one electron reaction with two protons transfer.The results also showed that the immobilized Hb keeped its electrocatalytic activity toward the reduction of O2.


hemoglobin(Hb), single-wall carbon nano-tube(SWCNT), didodecyldimethylamonium bromide(DDAB), direct electron transfer, cyclic voltammetry, electrochemical impedance spectroscopy

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