The adsorption and bioactivity of proteins at interfaces have been widely studied due to their important role in the construction of biosensors,bioelectronics and biofuel cells.Interfacial electric field is one of the important factors which could affect the adsorption and bioactivity of proteins at materials surfaces.It could dramatically change the adsorption density,molecular conformation and orientation at material surfaces.In this paper,the influence of interfacial electric field on the adsorption kinetics and bioactivity of hemoglobin(Hb) on a three-dimensional(3D) macroporous gold electrode surface has been studied using electrochemical methods and infrared spectroscopy.It was found that the interfacial electric field created excess surface charge which would accelerate the adsorption rate of Hb on the substrate by the enhanced electrostatic interactions between the electrode and protein patches.However,higher interfacial electric field could damage the conformation of the adsorbed Hb molecules,resulting in loss of the catalytic activity towards the reduction of hydrogen peroxide.Only at a surface with zero charge,the conformation and bioactivity of the adsorbed Hb molecules can be well retained.This work would provide fundamentals for the construction of biosensors,bioelectronics and biofuel cells,and assist to understand the interfacial behavior of biomolecules on charged biological interfaces.

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adsorption, protein, hemoglobin, charged surface, electric field, biocatalytic activity

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