Corresponding Author

Ai-lin LIU(ailinliu@mail.fjmu.edu.cn);
Xin-hua LIN(xinhua63@163.com)


A graphene modified glassy carbon electrode (GCE) was prepared by electrochemical reduction of graphite oxide fixed on the surface of GCE. Then, the amino-group modified probe of acute promyelocytic leukemia (APL) PML/RARα fusion gene fragment was immobilized onto surface of the graphene modified GCE by coupling activating agent. With methylene blue (MB) as a novel electrochemical indicator, artificial APL PML/RARα fusion gene fragment was measured by differential pulse voltammetry (DPV). The result indicated graphene nanomaterial showed good sensitization on the signal of MB. The relationship between the reduction peak current of MB and the concentration of complementary strand was linear in the range of 5×10-10~2.5×10-9 mol/L. The detection limit had been estimated as 8×10-11 mol/L. The method is simple, good specificity and high sensitivity. This approach is promising to the detection of APL in the real sample for medical diagnostics.

Graphical Abstract


Graphene, electrochemical DNA biosensor, PML/RAR&alpha, fusion gene

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