Corresponding Author

Chun-yan WANG(wangcy@ciac.ac.cn)


We developed a new method for electrochemical detection of hydrogen peroxide (H2O2) based on boronate oxidation of p-hydroxyphenylboronic acid. This method using p-aminophenol which is produced from the reaction of H2O2 and p-aminophenylboronic acid as a well electrochemical probe, combined with a gold nanoparticles (AuNPs) modified electrode for an indirect detection of H2O2. Because of the large surface area and enhanced electrocatalytic behavior by the AuNPs modified electrode, the detection sensitivity was improved. The method could detect H2O2 in the concentration range of 1 ~ 100 μmol•L-1 and 0.1 ~ 1 mmol•L-1 in 0.1 mol•L-1 pH 7.5 PBS containing 1.0 mmol•L-1 p-hydroxyphenylboronic acid. The low detection limit was 0.5 μmol•L-1. The proposed method had good selectivity and stability. Moreover, the method was quick, simple and cheap, which has potential application in real samples analysis.

Graphical Abstract


p-Hydroxyphenylboronic acid, hydrogen peroxide, gold nanoparticles, electrochemical detection

Publication Date


Online Available Date


Revised Date


Received Date



[1] Silva R A B, Montes R H O, Richter E M, et al. Rapid and selective determination of hydrogen peroxide residues in milk by batch injection analysis with amperometric detection[J]. Food Chemistry, 2012, 133(1): 200-204.

[2] Welch C M, Banks C E, Simm A O, et al. Silver nanoparticle assemblies supported on glassy-carbon electrodes for the electro-ananlytical detection of hydrogen peroxide[J]. Analytical and Bioanalytical Chemistry, 2005, 382(1): 12-21.

[3] Gao F X(高风仙), Yuan R(袁若), Chai Y Q(柴雅琴), et al. Hydrogen peroxide biosensor based on poly(thionine) and Au colloid[J]. Journal of Instrumental Analysis(分析测试学报), 2007, 26(1): 81-84.

[4] Li Y J, Huang F Y, Luo Z X, et al. A new hydrogen peroxide biosensor based on synergy of Au@Au2S2O3 core-shell nanomaterials and multi-walled carbon nanotubes towards hemoglobin[J]. Electrochimica Acta, 2012, 74: 280-286.

[5] Mao S X, Long Y M, Li W F, et al. Core-shell structured Ag@C for direct electrochemistry and hydrogen peroxide biosensor applications[J]. Biosensors and Bioelectronics, 2013, 48: 258-262.

[6] Tangkuaram T, Ponchio C, Kangkasomboon T, et al. Design and development of a highly stable hydrogen peroxide biosensor onscreen printed carbon electrode based on horseradish peroxidase bound with gold nanoparticles in the matrix of chitosan[J]. Biosensors and Bioelectronics, 2007, 22: 2071-2080.

[7] Li L(李理), LU H M(卢红梅), Deng Liu(邓留). H2O2 Electrochemistry biosensor based on graphene and gold nanorods composites[J]. Chinese Journal of Analytical Chemistry(分析化学), 2013, 41(5): 719-724.

[8] Miao Y E, He S X, Zhong Y L, et al. A novel hydrogen peroxide sensor based on Ag/SnO2 composite nanotubes by electrospinning[J]. Electrochimica Acta, 2013, 99: 117-123.

[9] Sun X L, Guo S J, Liu Y, et al. Dumbbell-like PtPd-Fe3O4 nanoparticles for enhanced electrochemical detection of H2O2[J]. Nano Letters, 2012, 12(9): 4859-4866.

[10] Huang J S, Wang D W, Hou H Q, et al. Electrospun palladium nanoparticle-loaded carbon nanofibers and their electrocatalytic activities towards hydrogen peroxide and NADH[J]. Advanced Functional Materials, 2008, 18(3): 441-448.

[11] Palanisamy S, Chen S M, Sarawathi R. A novel nonenzymatic hydrogen peroxide sensor based on reduced grapheme oxide/ZnO composite modi?ed electrode[J]. Sensors and Actuators B: Chemical, 2012, 166-167: 372-377.

[12] Gogoi A, Bora U. An iodine-promoted, mild and efficient method for the synthesis of phenols from arylboronic acids[J]. Synlett, 2012, 23(7): 1079-1081.

[13] Ci S Q(次素琴), Zhan L(战磊), Zou J P(邹建平), et al. Electrocatalytic activity and detection of dihydroxybenzenes on porous carbon loading polymerized phthalocyanatocobalt modified electrode[J]. Chinese Journal of Analytical Chemistry(分析化学), 2013, 41(8): 1238-1242.

[14] Debdeep M, Rajeev G, Ravi G, et al. Calix[4]arene functionalized gold nanoparticles: Application incolorimetric and electrochemical sensing of cobalt ion in organic and aqueous medium[J]. Sensors and Actuators B: Chemical, 2014, 191: 757-764.

[15] Singh S, Jain D V S, Singla M L. Sol-gel based composite of gold nanoparticles as matix for tyrosinase for amperometric catechol biosensor[J]. Sensors and Actuators B: Chemical, 2013, 182: 161-169.

[16] Grabar K C, Freeman R G, Hommer M B, et al. Preparation and characterization of Au colloid monolayers[J]. Analytical Chemistry, 1995, 67(4): 735-743.

[17] Chowdhury A D, Mobin S M, Mukherjee S. [Pd(L)Cl2]-catalyzed selective hydroxylation of arylboronic acids to phenols[J]. European Journal of Inorganic Chemistry, 2011, 21: 3232-3239.

[18] Wu P(吴萍), Cai C X(蔡称心). Horseradish peroxidase-attapulgite clay nanocomposites: Fabrication and application to sensing the extracellular H2O2 released from cells[J]. Journal of Electrochemistry(电化学), 2014, 20(3): 260-265.

[19] Jia J B, Wang B Q, Wu A G, et al. A method to construct a third-generation horseradish peroxidase biosensor: Self-assembling gold nanoparticles to three-dimensional sol-gel network[J]. Analytical Chemistry, 2002, 74(9): 2217-2223.

[20] Fan L L(范丽丽), Wu L N(武丽娜), Qu Z Y(屈志宇), et al. Preparation of Pt/DNA-MWCNTs/GC electrode and its electrocatalytic activity toward H2O2 reduction[J]. Journal of Electrochemistry(电化学), 2014, 20(5): 459-464.



To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.