Corresponding Author

You-jun FAN(youjunfan@mailbox.gxnu.edu.cn)


With a glassy carbon electrode (GCE) as the substrate, the poly(3,4-ethylenedioxythiophene) (PEDOT) film electrode was prepared through the electrochemical polymerization method, then a novel non-enzymatic electrochemical H2O2 sensor was fabricated by co-immobilizing phosphomolybdic acid and graphene with Nafion on the PEDOT/GCE electrode. The modified electrodes were characterized by scanning electron microscopy (SEM), while the responsive properties of the sensor to H2O2 were investigated by cyclic voltammetry and chronoamperometry. The results demonstrated that, under the optimized conditions, the sensor exhibited good electrocatalytic performance for H2O2 reduction. The current response of the sensor was linear to the H2O2 concentration in the range of 2.91×10-6 ~ 1.83×10-2 mol•L-1 with the detection limit and sensitivity being 9.90×10-7 mol•L-1 (S/N = 3) and 112.5 μA•(mmol•L-1)-1, respectively. Moreover, the sensor also exhibited excellent reproducibility and selectivity.

Graphical Abstract


electrochemical H2O2 sensor, poly(3, 4-ethylenedioxythiophene), phosphmolybdic acid, graphene

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[1] Wang L, Zhang Y Y, Cheng C S, et al. Highly sensitive electrochemical biosensor for evaluation of oxidative stress based on the nanointerface of graphene nanocomposites blended with gold, Fe3O4, and platinum nanoparticles[J]. ACS Applied Materials & Interfaces, 2015, 7(33): 18441-18449.

[2] Fan L L(范丽丽), Wu L N(武丽娜), Qu Z Y(屈志宇), et al. Preparation of Pt/DNA-MWCNTs/GC electrode and its electrocatalytic activity for H2O2 reduction[J]. Journal of Electrochemistry(电化学), 2014, 20(5): 459-464.

[3] Emre F B, Ekiz F, Balan A, et al. Conducting polymers with benzothiadiazole and benzoselenadiazole- units for biosensor applications[J]. Sensors and Actuators B, 2011, 158(1): 117-123.

[4] Ates M, Sarac S A. Conducting polymer coated carbon surfaces and biosensor applications[J]. Progress in Organic Coating, 2009, 66(4): 337-358.

[5] Lin K C, Tsai T H, Chen S M. Performing enzyme-free H2O2 biosensor and simultaneous determination for AA, DA, and UA by MWCNT-PEDOT film[J]. Biosensors and Bioelectronics, 2010, 26(2): 608-614.

[6] Xu J J, Peng R, Ran Q, et al. A highly soluble poly(3,4-ethylenedioxythiophene)-poly(styrene sulfonic-acid)/Au nanocomposite for horseradish peroxidase immobilization and biosensing[J]. Talanta, 2010, 82(4): 1511-1515.

[7] Rozlosnik N. New directions in medical biosensors employing poly(3,4-ethylenedioxythiophene) derivative-based electrodes[J]. Analytical and Bioanalytical Chemistry, 2009, 395(3): 637-645.

[8] Liu M M, Liu R, Chen W. Graphene wrapped Cu2O nanocubes: Non-enzymatic electrochemical sensors for the detection of glucose and hydrogen peroxide with enhanced stability[J]. Biosensors and Bioelectronics, 2013, 45: 206-212.

[9] Pumera M, Ambrosi A, Bonanni A, et al. Graphene for electrochemical sensing and biosensing [J]. TrAC Trends in Analytical Chemistry, 2010, 29(9): 954-965.

[10] Ma J H(马静华), Wang R X(王睿翔), Tan Y L(谭一良), et al. Preparation and methanol electrooxidation of Pt/PMo12/PEDOT/GC electrodes[J]. Journal of Electrochemistry(电化学), 2013, 19(2): 164-168.

[11] He X, Chen L, Zhang X, et al. Enhanced electrocatalysis for methanol oxidation on ordered {[PdPW11O39]5-/Pt/PAMAM}n multilayer composites[J]. Fuel Cells, 2015, 15(1): 221-229.

[12] Genovese M, Foong Y W, Lian K. Designing polyoxometalate based layer-by-layer thin films on carbon nanomaterials for pseudocapacitive electrodes[J]. Journal of the Electrochemical Society, 2015, 162(5): 5041-5046.

[13] Guo Z P, Han D M, Wexler D, et al. Polyoxometallate-stabilized platinum catalysts on multi-walled carbon nanotubes for fuel cell applications[J]. Electrochimica Acta, 2008, 53(22): 6410-6416.

[14] Zhu L L(朱莉莉), Qu J Y(屈建莹), Wang J(王珏), et al. Electrochemical properties of tungstogermanate and electrocatalysis of H2O2 on GeW11Co/P-oPD/GC electrode [J]. Journal of Electrochemistry(电化学), 2011, 17(4): 410-415.

[15] Skunik M, Kulesza P J. Phosphomolybdate-modified multi-walled carbon nanotubes as effective mediating systems for electrocatalytic reduction of bromate[J]. Analytica Chimica Acta, 2009, 631(2): 153-160.

[16] Wlodarczyk R, Chojak M, Miecznikowski K, et al. Electroreduction of oxygen at polyoxometallate-modified glassy carbon-supported Pt nanoparticles[J]. Journal of Power Sources, 2006, 159(2): 802-809.

[17] Fan L L(范丽丽), Fan Y J(樊友军), Wang S S(王珊珊), et al. H2O2 biosensor based on Pt/PEDOT/PSS-graphene composite[J]. Chinese Journal of Sensors and Actuators(传感技术学报), 2013, 26(4): 446-451.

[18] Nagashree K L, Raviraj N H, Ahmed M F. Carbon paste electrodes modified by Pt and Pt-Ni microparticles dispersed in polyindole film for electrocatalytic oxidation of methanol[J]. Electrochimica Acta, 2010, 55: 2629-2635.

[19] Yin H S, Ai S Y, Shi W J, et al. A novel hydrogen peroxide biosensor based on horseradish peroxidase immobilized on gold nanoparticles-silk fibroin modified glassy carbon electrode and direct electrochemistry of horseradish peroxidase[J]. Sensors and Actuators B, 2009, 137(2): 747-753.

[20] Miao Y Q, Wang H, Shao Y Y, et al. Layer-by-layer assembled hybrid film of carbon nanotubes/iron oxide nanocrystals for reagentless electrochemical detection of H2O2[J]. Sensors and Actuators B, 2009, 138(1): 182-188.

[21] Chen C C, Do J S, Gu Y S. Immobilization of HRP in mesoporous silica and its application for the construction of polyaniline modified hydrogen peroxide[J]. Sensors, 2009, 9(6): 4635-4648.

[22] Tan X C, Zhang J L, Tan S W, et al. Amperometric hydrogen peroxide biosensor based on Immobilization of hemoglobin on a glassy carbon electrode modified with Fe3O4/chitosan core-shell microspheres[J]. Sensors, 2009, 9(8): 6185-6199.

[23] Wang K, Liu Q, Wu X Y, et al. Graphene enhanced electrochemiluminescence of CdS nanocrystal for H2O2 sensing[J]. Talanta, 2010, 82(1): 372-376.

[24] Cui X L, Li Z Z, Yang Y C, et al. Low-potential sensitive hydrogen peroxide detection based on nanotubular TiO2 and platinum composite electrode[J]. Electroanalysis, 2008, 20(9): 970-975.

[25] Shamsipur M, Asgari M, Mousavi M F, et al. A novel hydrogen peroxide sensor based on the direct electron transfer of catalase immobilized on nano-sized NiO/MWCNTs composite film[J]. Electroanalysis, 2012, 24(2): 357-367.

[26] Li S, Xiong J X, Shen J S, et al. A novel hydrogen peroxide sensor based on Ag nanoparticles decorated polyaniline/graphene composites[J]. Journal of Applied Polymer Science, 2015, 132(37): 42409/1-6.



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