Simultaneous Determinations of Ascorbic Acid, Dopamine and Uric Acid at CA/GC Electrode

Authors

Ying ZHANG, College of Chemical & Pharmaceutical Engineering, Sichuan University of Science & Engineering, Zigong, Sichuan 643000, China;
Wang REN, College of Chemical & Pharmaceutical Engineering, Sichuan University of Science & Engineering, Zigong, Sichuan 643000, China;
Min-Jiao LI, College of Chemical & Pharmaceutical Engineering, Sichuan University of Science & Engineering, Zigong, Sichuan 643000, China;

Corresponding Author

Ying ZHANG

Abstract

The citric acid modified glassy carbon electrode (CA/GC) was constructed by potentiostatic technique and used for simultaneous determinations of ascorbic acid (AA), dopamine (DA) and uric acid (UA) by cyclic voltammetry (CV) and differential pulse voltammetry (DPV). The CA/GC electrode exhibits excellent electrocatalytic activity towards AA, DA and UA. The oxidation peaks are well separated with the potential differences between AA and DA, DA and UA, and AA and UA being 170, 130 and 300 mV, respectively, which is large enough to determine AA, DA and UA simultaneously. The catalytic peak currents obtained were linearly dependent on the concentrations of AA, DA and UA in the range of 2.0×10-6~1.5×10-3 mol?L-1, 6.0×10-7~1.0×10-3 mol?L-1, and 6.0×10-7~1.0×10-3 mol?L-1, respectively. The proposed sensor shows good reproducibility and may be applied practically in the determinations of AA, DA and UA.

Graphical Abstract

Keywords

ascorbic acid, dopamine, uric acid, citric acid, modified electrode

DOI Link

https://doi.org/10.61558/2993-074X.2884

Publication Date

2012-02-28

Online Available Date

2011-09-29

Revised Date

2011-09-02

Received Date

2011-08-22

Recommended Citation

Ying ZHANG, Wang REN, Min-Jiao LI. Simultaneous Determinations of Ascorbic Acid, Dopamine and Uric Acid at CA/GC Electrode[J]. Journal of Electrochemistry, 2012 , 18(1): Article 13.
DOI: 10.61558/2993-074X.2884
Available at: https://jelectrochem.xmu.edu.cn/journal/vol18/iss1/13

References

[1] Arrigoni O, Tullio C D. Ascorbic acid: more than just an antioxidant. Biochim. Biophys. Acta, 2002, 11569: 1-9.

[2] Li N B, Ren W, Luo H Q. Caffeic Acid-Modified Glassy Carbon Electrode for the Simultaneous Determination of Epinephrine and Dopamine[J]. Electroanalysis, 2007, 19: 1496-1502.

[3] Kaur H, Halliwell B. Action of biologically-relevant oxidizing species upon uric acid. Identi?cation of uric acid oxidation products[J]. Chem. Biol. Interact. 1990, 73: 235-247.

[4] Ren W, Luo H Q, Li N B Simultaneous voltammetric measurement of ascorbic acid, epinephrine and uric acid at a glassy carbon electrode modified with caffeic acid[J]. Biosens. Bioelectronics, 2006, 21: 1086-1092.


[5] Li N B, Ren W, Luo H Q. Simultaneous voltammetric measurement of ascorbic acid and dopamine on poly(caffeic acid)-modified glassy carbon electrode[J]. J Solid State Electrochem, 2008, 12: 693-699.

[6] Yuan Y, Saleh Ahammad A J, Xu G R, et al. Poly(thionine)-modified GC Electrode for Simultaneous Detection of Dopamine and Uric Acid in the Presence of Ascorbic Acid[J]. Bull. Korean Chem. Soc. 2008, 29, 1883-1884.

[7] Ensafi, Ali A, Taei, M, Khayamian, T. Highly selective determination of ascorbic acid, dopamine, and uric acid by differential pulse voltammetry using poly(sulfonazo III) modified glassy carbon electrode[J]. Sensors Actuators, B: Chemi, 2010, B147: 213-221.

[8] Ren W, Zhang Y, Li M J. Simultaneous Determination of Dopamine (DA) and Epinephrine (EP) at CA/GC Electrode[J]. J Electrochem, 2011 (3): 343-346.

[9] Wan F L, Zhang W, Gu J. Determination of tetrahydrobiopterin and other relatedcompounds by Mn-doped PbO2 modified electrode[J]. CHEMI SENSORS, 2003, 23 (4): 15-22.