The Electrochemical Properties of 1-Pyrenebutyric acid/Graphene Composites and Their Application in Glucose Biosensors

Authors

Min Wang, State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China;
Jiong Wang, State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China;
Fengbin Wang, State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China;
Xinghua Xia, State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China;Follow

Corresponding Author

Xinghua Xia(xhxia@nju.edu.cn)

Abstract

The electrochemical properties of 1-pyrenebutyric acid/graphene composites (PBA/G) obtained by one-step synthesis via π-π stacking was investigated. The electrochemical impedance titration curve shows the surface charge changes as function of solution pH by using ferricyanide/ferrocyanide redox couple as the probe. An apparent pK_a value is estimated as 6.2 according to the impedance titration curve. In addition, a glucose biosensor was constructed by immobilizing glucose oxidase (GOD) on the surface of PBA/G via covalent interaction. This biosensor shows a linear response to glucose within the concentration up to 5 mmol L^-1 with a detection limit of 0.085 mmol L^-1. A small apparent Michaelis-Menten constant (5.40 mmol L^-1) of the immobilized GOD suggests that the immobilized GOD retains its bioactivity and shows high catalytic activity to glucose.

Graphical Abstract

Keywords

1-pyrenebutyric acid, graphene, glucose oxidase, electrochemical biosensor, glucose

DOI Link

https://doi.org/10.61558/2993-074X.2615

Publication Date

2012-10-28

Online Available Date

2012-08-18

Revised Date

2012-08-16

Received Date

2012-06-26

Recommended Citation

Min Wang, Jiong Wang, Fengbin Wang, Xinghua Xia. The Electrochemical Properties of 1-Pyrenebutyric acid/Graphene Composites and Their Application in Glucose Biosensors[J]. Journal of Electrochemistry, 2012 , 18(5): Article 7.
DOI: 10.61558/2993-074X.2615
Available at: https://jelectrochem.xmu.edu.cn/journal/vol18/iss5/7

References

[1] Stoller M D, Park S, Zhu Y W, et al. Graphene-based ultracapacitors[J]. Nano Letters, 2008, 8(10): 3498-3502.

[2] Schedin F, Geim A K, Morozov S V, et al. Detection of individual gas molecules adsorbed on graphene[J]. Nature Materials, 2007, 6(9): 652-655.

[3] Zhou M, Zhai Y M, Dong S J. Electrochemical sensing and biosensing platform based on chemically reduced graphene oxide[J]. Analytical Chemistry, 2009, 81(14): 5603-5613.

[4] Lee H, Ihm J, Cohen M L, et al. Calcium-decorated graphene-based nanostructures for hydrogen storage[J]. Nano Letters, 2010, 10(3): 793-798.

[5] Gilje S, Han S, Wang M S, et al. A chemical route to graphene for device applications[J]. Nano Letters, 2007, 7(11): 3394-3398.

[6] Park S, Ruoff R S. Chemical methods for the production of graphenes[J]. Nature Nanotechnology, 2009, 4(4): 217-224.

[7] Shinde D B, Debgupta J, Kushwaha A, et al. Electrochemical unzipping of multi-walled carbon nanotubes for facile synthesis of high-quality graphene nanoribbons[J]. Journal of the American Chemical Society, 2011, 133(12): 4168-4171.

[8] Zeng Q, Cheng J S, Tang L H, et al. Self-assembled graphene-enzyme hierarchical nanostructures for electrochemical biosensing[J]. Advanced Functional Materials, 2010, 20(19): 3366-3372.

[9] Zhang Q, Qiao Y, Hao F, et al. Fabrication of a biocompatible and conductive platform based on a single-stranded DNA/graphene nanocomposite for direct electrochemistry and electrocatalysis[J]. Chemistry-A European Journal, 2010, 16(27): 8133-8139.

[10] Kang X H, Wang J, Wu H, et al. Glucose oxidase-graphene-chitosan modified electrode for direct electrochemistry and glucose sensing[J]. Biosensors and Bioelectronics, 2009, 25(4): 901-905.

[11] Wang M, Xiao F N, Wang K, et al. Electric field driven protonation/deprotonation of 3,4,9,10-perylene tetracarboxylic acid immobilized on graphene sheets via π-π stacking[J]. Journal of Electroanalytical Chemistry, 2012, in press: doi: http://dx.doi.org/10.1016/j.jelechem.2012.07.036.

[12] Wang Y, Shao Y Y, Matson D W, et al. Nitrogen-doped graphene and its application in electrochemical biosensing[J]. ACS Nano, 2010, 4(4): 1790-1798.

[13] Chen D, Tang L, Li J H. Graphene-based materials in electrochemistry[J]. Chemical Society Review, 2010, 39(8): 3157-3180.

[14] Wang Y, Li Z H, Wang J, et al. Graphene and graphene oxide: Biofunctionalization and applications in biotechnology[J]. Trends in Biotechnology, 2011, 29(5): 205-212.

[15] Zhang Q, Wu S, Zhang L, et al. Fabrication of polymeric ionic liquid/graphene nanocomposite for glucose oxidase immobilization and direct electrochemistry[J]. Biosensors and Bioelectronics, 2011, 26(5): 2632-2637.

[16] Chen S H, Duhamel J, Bahun G J, et al. Quantifying the presence of unwanted fluorescent species in the study of pyrene-labeled macromolecules[J]. Journal of Physical Chemistry B, 2011, 115(33): 9921-9929.

[17] Chen R J, Zhang Y G, Wang D W, et al. Noncovalent sidewall functionalization of single-walled carbon nanotubes for protein immobilization[J]. Journal of the American Chemical Society, 2001, 123(16): 3838-3839.

[18] Xu Y X, Bai H, Lu G W, et al. Flexible graphene films via the filtration of water-soluble noncovalent functionalized graphene sheets[J]. Journal of the American Chemical Society, 2008, 130(18): 5856-5857.

[19] Gao W C, Dong H F, Lei J P, et al. Signal amplification of streptavidin-horseradish peroxidase functionalized carbon nanotubes for amperometric detection of attomolar DNA[J]. Chemical Communications, 2011, 47: 5220-5222.

[20] Liu F, Choi K S, Park T J, et al. Graphene-based electrochemical biosensor for pathogenic virus detection[J]. BioChip Journal, 2011, 5(2): 123-128.

[21] Guo H L, Wang X F, Qian Q Y, et al. A green approach to the synthesis of graphene nanosheets[J]. ACS Nano, 2009, 3(9): 2653-2659.

[22] Guldi D M, Rahman G M A, Jux N, et al. Functional single-wall carbon nanotube nanohybrids associating SWNTs with water-soluble enzyme model systems[J]. Journal of the American Chemical Society, 2005, 127(27): 9830-9838.

[23] Zhao J W, Luo L Q, Yang X R, et al. Determination of surface pKa of SAM using an electrochemical titration method[J]. Electroanalysis, 1999, 11(15): 1108-1113.

[24] Tulock J J, Blanchard G J. Role of probe molecule structure in sensing solution phase interactions in ternary systems[J]. The Journal of Physical Chemistry A, 2000, 104(36): 8340-8345.

[25] Hu L Z, Han S, Liu Z Y, et al. A versatile strategy for electrochemical detection of hydrogen peroxide as well as related enzymes and substrates based on selective hydrogen peroxide-mediated boronate deprotection[J]. Electrochemistry Communications, 2011, 13(12): 1536-1538.

[26] Jia W Z, Wang K, Zhu Z J, et al. One-step immobilization of glucose oxidase in a silica matrix on a Pt electrode by an electrochemically induced sol-gel process[J]. Langmuir, 2007, 23(23): 11896-11900.