The Electrochemical Properties of 1-Pyrenebutyric acid/Graphene Composites and Their Application in Glucose Biosensors

Authors

Min Wang, State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China;
Jiong Wang, State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China;
Fengbin Wang, State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China;
Xinghua Xia, State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China;Follow

Corresponding Author

Xinghua Xia(xhxia@nju.edu.cn)

Abstract

The electrochemical properties of 1-pyrenebutyric acid/graphene composites (PBA/G) obtained by one-step synthesis via π-π stacking was investigated. The electrochemical impedance titration curve shows the surface charge changes as function of solution pH by using ferricyanide/ferrocyanide redox couple as the probe. An apparent pK_a value is estimated as 6.2 according to the impedance titration curve. In addition, a glucose biosensor was constructed by immobilizing glucose oxidase (GOD) on the surface of PBA/G via covalent interaction. This biosensor shows a linear response to glucose within the concentration up to 5 mmol L^-1 with a detection limit of 0.085 mmol L^-1. A small apparent Michaelis-Menten constant (5.40 mmol L^-1) of the immobilized GOD suggests that the immobilized GOD retains its bioactivity and shows high catalytic activity to glucose.

Graphical Abstract

Keywords

1-pyrenebutyric acid, graphene, glucose oxidase, electrochemical biosensor, glucose

DOI Link

https://doi.org/10.61558/2993-074X.2615

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Publication Date

2012-10-28

Online Available Date

2012-08-18

Revised Date

2012-08-16

Received Date

2012-06-26

Recommended Citation

Min Wang, Jiong Wang, Fengbin Wang, Xinghua Xia. The Electrochemical Properties of 1-Pyrenebutyric acid/Graphene Composites and Their Application in Glucose Biosensors[J]. Journal of Electrochemistry, 2012 , 18(5): Article 7.
DOI: 10.61558/2993-074X.2615
Available at: https://jelectrochem.xmu.edu.cn/journal/vol18/iss5/7