Abstract
The effects of pH, ligtlnd CN￣-,metallic ions,Hg￣(+2), Cd￣(+2) and Pb￣(2+) on electroactivity of cytochrome c have been studied with cyclic voltammetry and UV/Vis spectroseopy.In the phosphate buffer solution containing 5 mg/mL cytochrome c, cytochrome c is electroactive at a glod electrode with suitable pretreatment if the pH value is equal to 7.0, but it is electroinactive at the gold electrode if the pH value is equal to 2. 0 or 10.0. The existence of CN- ions in the solution would cause deactivation of cytochrome c in the bulk sulurion, and would not affect the electroactivity of cytochrome c adsorbed on the gold electrode。The sequence for effects of metallic ions on electroactivity of cytochrome c is Hg￣(+2)>Cd￣(+2)>Pb￣(2+).The possible mechanisms were proposed.
Keywords
Cytochrome c, pH, Ligand, Metallic ions
Publication Date
1996-02-28
Online Available Date
1996-02-28
Revised Date
1996-02-28
Received Date
1996-02-28
Recommended Citation
Jin Luo, Lingling Wu, Jintain Wu, Shaohua Huang, Zhonghua Lin.
Effects of pH,Ligand CN-,Metallic Ions Hg￣(+2),Cd￣(+2) and Pb￣(2+) on Electroactivity of Cytochrome C[J]. Journal of Electrochemistry,
1996
,
2(1): 61-65.
DOI: 10.61558/2993-074X.1327
Available at:
https://jelectrochem.xmu.edu.cn/journal/vol2/iss1/4
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