Effects of pH, Ligand CN^-, Metallic Ions Hg²⁺, Cd²⁺ and Pb²⁺ on Electroactivity of Cytochrome C

Authors

Jin Luo, State Key Lab. for Phys. Chem. of the Solid Surf., Dept. of Chem., Xiamen Univ., Xiamen 361005
Lingling Wu, State Key Lab. for Phys. Chem. of the Solid Surf., Dept. of Chem., Xiamen Univ., Xiamen 361005
Jintain Wu, State Key Lab. for Phys. Chem. of the Solid Surf., Dept. of Chem., Xiamen Univ., Xiamen 361005
Shaohua Huang, State Key Lab. for Phys. Chem. of the Solid Surf., Dept. of Chem., Xiamen Univ., Xiamen 361005
Zhonghua Lin, State Key Lab. for Phys. Chem. of the Solid Surf., Dept. of Chem., Xiamen Univ., Xiamen 361005

Corresponding Author

Jin Luo

Abstract

The effects of pH, ligtlnd CN^-, metallic ions, Hg²⁺, Cd²⁺ and Pb²⁺ on electroactivity of cytochrome c have been studied with cyclic voltammetry and UV/Vis spectroseopy. In the phosphate buffer solution containing 5 mg/mL cytochrome c, cytochrome c is electroactive at a glod electrode with suitable pretreatment if the pH value is equal to 7.0, but it is electroinactive at the gold electrode if the pH value is equal to 2.0 or 10.0. The existence of CN^- ions in the solution would cause deactivation of cytochrome c in the bulk sulurion, and would not affect the electroactivity of cytochrome c adsorbed on the gold electrode. The sequence for effects of metallic ions on electroactivity of cytochrome c is Hg²⁺>Cd²⁺>Pb²⁺. The possible mechanisms were proposed.

Keywords

Cytochrome c, pH, Ligand, Metallic ions

DOI Link

https://doi.org/10.61558/2993-074X.1327

Publication Date

1996-02-28

Online Available Date

1996-02-28

Revised Date

1995-09-29

Received Date

1995-07-29

Recommended Citation

Jin Luo, Lingling Wu, Jintain Wu, Shaohua Huang, Zhonghua Lin. Effects of pH, Ligand CN^-, Metallic Ions Hg²⁺, Cd²⁺ and Pb²⁺ on Electroactivity of Cytochrome C[J]. Journal of Electrochemistry, 1996 , 2(1): 61-65.
DOI: 10.61558/2993-074X.1327
Available at: https://jelectrochem.xmu.edu.cn/journal/vol2/iss1/4