Abstract
In this paper, a novel electrochemiluminescence (ECL) immunosensor for the detection of apolipoprotein A1 was constructed based on flower-like platinum nanoparticles (PtNFs) via a one-pot chemical synthesis method. The PtNFs was used to immobilize the secondary antibody and enzyme (GOD). Then the prepared bioconjugates were introduced onto the electrode via sandwich immunoreactions. Accordingly, the ECL luminophore peroxydisulfate (S2O82- ) was presented in the working buffer solution containing an appropriate amount of glucose. Through the ECL responses of S2O82- and O2, a dramatically amplified ECL signal was obtained for the reason that hydrogen peroxide (H2O2) produced by GOD to glucose was subsequently catalyzed by PtNFs to in situ generate O2. The present immunosensor showed a wide linear range of 0.1 ng•mL-1 to 100 ng•mL-1, with a low detection limit of 0.03 ng•mL-1 for detecting Apo-A1. This new signal amplification strategy for preparation of ECL immunosensor could be easily realized and has potential application in ultrasensitive bioassays.
Graphical Abstract
Keywords
flower-like structures platinum nanoparticles, electrochemiluminescence immunosensor, apolipoprotein A1
Publication Date
2016-06-28
Online Available Date
2015-11-18
Revised Date
2015-11-10
Received Date
2015-09-14
Recommended Citation
LIAO Ni, ZHUO Ying, YUAN Ruo.
Electrochemiluminescence Immunosensor Based on Platinum Nanoparticles for the Determination of Apolipoprotein A1[J]. Journal of Electrochemistry,
2016
,
22(3): 299-305.
DOI: 10.13208/j.electrochem.150914
Available at:
https://jelectrochem.xmu.edu.cn/journal/vol22/iss3/9
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