Electrochemiluminescence Immunosensor Based on Platinum Nanoparticles for the Determination of Apolipoprotein A1

Authors

LIAO Ni, College of Biological and Chemical Engineering, Panzhihua University, Panzhihua 617000, Sichuan, China;Follow
ZHUO Ying, Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China;
YUAN Ruo, Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China;

Corresponding Author

LIAO Ni(liaoni123456789@163.com)

Abstract

In this paper, a novel electrochemiluminescence (ECL) immunosensor for the detection of apolipoprotein A1 was constructed based on flower-like platinum nanoparticles (PtNFs) via a one-pot chemical synthesis method. The PtNFs was used to immobilize the secondary antibody and enzyme (GOD). Then the prepared bioconjugates were introduced onto the electrode via sandwich immunoreactions. Accordingly, the ECL luminophore peroxydisulfate (S₂O₈^2- ) was presented in the working buffer solution containing an appropriate amount of glucose. Through the ECL responses of S₂O₈^2- and O₂, a dramatically amplified ECL signal was obtained for the reason that hydrogen peroxide (H₂O₂) produced by GOD to glucose was subsequently catalyzed by PtNFs to in situ generate O₂. The present immunosensor showed a wide linear range of 0.1 ng•mL^-1 to 100 ng•mL^-1, with a low detection limit of 0.03 ng•mL^-1 for detecting Apo-A1. This new signal amplification strategy for preparation of ECL immunosensor could be easily realized and has potential application in ultrasensitive bioassays.

Graphical Abstract

Keywords

flower-like structures platinum nanoparticles, electrochemiluminescence immunosensor, apolipoprotein A1

DOI Link

https://doi.org/10.13208/j.electrochem.150914

Publication Date

2016-06-28

Online Available Date

2015-11-18

Revised Date

2015-11-10

Received Date

2015-09-14

Recommended Citation

LIAO Ni, ZHUO Ying, YUAN Ruo. Electrochemiluminescence Immunosensor Based on Platinum Nanoparticles for the Determination of Apolipoprotein A1[J]. Journal of Electrochemistry, 2016 , 22(3): 299-305.
DOI: 10.13208/j.electrochem.150914
Available at: https://jelectrochem.xmu.edu.cn/journal/vol22/iss3/9

References

[1] Zhang Z T, Pak J, Huang H Y, et al. Role of Ha-ras activation in superficial papillary pathway of urothelial tumor formation[J]. Oncogene, 2001, 20(12): 1973-1980.

[2] Chen Y T, Chen C L, Chen H W, et al. Discovery of novel bladder cancer biomarkers by comparative urine proteomics using iTRAQ technology[J]. Journal of Proteome Research, 2010, 9(11): 5803-5815.

[3] Li C Y, Li H J, Li J M, et al. Discovery of Apo-A1 as a potential bladder cancer biomarker by urine proteomics and analysis[J]. Biochemical and Biophysical Research Communications, 2014, 4(446): 1047-1052.

[4] Li H J, Li C Y, Wu H L, et al. Identification of Apo-A1 as a biomarker for early diagnosis of bladder transitional cell carcinoma[J]. Proteome Science, 2011, 9: 21-23.

[5] Wu Y, Shi H, Yuan L. A novel electrochemiluminescence immunosensor via polymerization-assisted amplification[J]. Chemical Communications, 2010, 46(41): 7763-7765.

[6] Zanarini S, Rampazzo E, Ciana L D. Ru(bpy)₃ covalently doped silica nanoparticles as multicenter tunable structures for electrochemiluminescence amplification[J]. Journal of the American Chemical Society, 2009, 131(6): 2260-2267.

[7] Chen Z H, Liu Y, Wang Y Z, et al. Dynamic evaluation of cell surface N-Glycan expression via an electrogenerated chemiluminescence biosensor based on concanavalin A-Integrating gold-nanoparticle-modified Ru(bpy)₃²⁺-Doped silica nanoprobe[J]. Analytical Chemistry, 2013, 85(9): 4431-4438.

[8] Hu L Z, Xu G B. Applications and trends in electrochemiluminescence[J]. Chemical Society Reviews, 2010, 39(8): 3275-3304.

[9] Chen X M, Wu G H, Chen J M, et al. A novel electrochemiluminescence sensor based on bis(2,2€²-bipyridine)-5-amino-1,10-phenanthroline ruthenium(II) covalently combined with graphite oxide[J]. Biosensors and Bioelectronics, 2010, 26(2): 872-876.

[10] Tang D and Ren J. In situ amplified electrochemical immunoassay for carcinoembryonic antigen using horseradish peroxidase-encapsulated nanogold hollow microspheres as labels[J]. Analytical Chemistry, 2008, 80(21): 8064-8070.

[11] Jian H and Ju H. Enzyme-quantum dots architecture for highly sensitive electrochemiluminescence biosensing of oxidase substrates[J]. Chemical Communications, 2007: 404-406.

[12] Liu X, Ju H. Coreactant enhanced anodic electrochemiluminescence of CdTe quantum dots at low potential for sensitive biosensing amplified by enzymatic cycle[J]. Analytical Chemistry, 2008, 80(14): 5377-5382.

[13] Liu X, Zhang Y, Lei J. Quantum dots based electrochemiluminescent immunosensor by coupling enzymatic amplification with self-produced coreactant from oxygen reduction[J]. Analytical Chemistry, 2010, 82(17): 7351-7356.

[14] Qiu B, Lin Z, Wang J. An electrochemiluminescent biosensor for glucose based on the electrochemiluminescence of luminol on the nafion/glucose oxidase/poly(nickel(II) tetrasulfophthalocyanine)/multi-walled carbon nanotubes modified electrode[J]. Talanta, 2009, 78(1): 76-80.

[15] Liu X, Niu W, Li H. Glucose biosensor based on gold nanoparticle-catalyzed luminol electrochemiluminescence on a three-dimensional sol-gel network[J]. Electrochemistry Communications, 2008, 10(9): 1250-1253.

[16] Zhuo Y, Yuan P, Yuan R, et al. Bienzyme functionalized three-layer composite magnetic nanoparticles for electrochemical immunosensors[J]. Biomaterials, 2009, 30(12): 2284-2290.

[17] Niu H, Yuan R, Chai Y, et al. Electrochemiluminescence of peroxydisulfate enhanced by L-Cysteine film for sensitive immunoassay[J]. Biosensors and Bioelectronics, 2011, 26(7): 3175-3180.

[18] Lei Y M, Huang W X, Yuan R, et al. Electrochemiluminescence resonance energy transfer system: Mechanism and application in ratiometric aptasensor for lead ion[J]. Analytical Chemistry, 2015, 87(15): 7787-7794.

[19] Lv X H, Pang X H, Li Y Y, et al. Electrochemiluminescent immune-modified electrodes based on Ag₂Se@CdSe nanoneedles loaded with polypyrrole intercalated graphene for detection of CA72-4[J]. ACS Applied Materials & Interfaces, 2015, 7(1): 867-872.

[20]Reshetnyak O V, Koval`chuk E P, Skurski P, et al. The origin of luminescence accompanying electrochemical reduction or chemical decomposition of peroxydisulfates[J]. Journal of Luminescence, 2003, 105(1): 27-34.