Electrochemiluminescence Immunosensor Based on Platinum Nanoparticles for the Determination of Apolipoprotein A1

Authors

LIAO Ni, College of Biological and Chemical Engineering, Panzhihua University, Panzhihua 617000, Sichuan, China;Follow
ZHUO Ying, Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China;
YUAN Ruo, Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China;

Corresponding Author

LIAO Ni(liaoni123456789@163.com)

Abstract

In this paper, a novel electrochemiluminescence (ECL) immunosensor for the detection of apolipoprotein A1 was constructed based on flower-like platinum nanoparticles (PtNFs) via a one-pot chemical synthesis method. The PtNFs was used to immobilize the secondary antibody and enzyme (GOD). Then the prepared bioconjugates were introduced onto the electrode via sandwich immunoreactions. Accordingly, the ECL luminophore peroxydisulfate (S₂O₈^2- ) was presented in the working buffer solution containing an appropriate amount of glucose. Through the ECL responses of S₂O₈^2- and O₂, a dramatically amplified ECL signal was obtained for the reason that hydrogen peroxide (H₂O₂) produced by GOD to glucose was subsequently catalyzed by PtNFs to in situ generate O₂. The present immunosensor showed a wide linear range of 0.1 ng•mL^-1 to 100 ng•mL^-1, with a low detection limit of 0.03 ng•mL^-1 for detecting Apo-A1. This new signal amplification strategy for preparation of ECL immunosensor could be easily realized and has potential application in ultrasensitive bioassays.

Graphical Abstract

Keywords

flower-like structures platinum nanoparticles, electrochemiluminescence immunosensor, apolipoprotein A1

DOI Link

https://doi.org/10.13208/j.electrochem.150914

Publication Date

2016-06-28

Online Available Date

2015-11-18

Revised Date

2015-11-10

Received Date

2015-09-14

Recommended Citation

LIAO Ni, ZHUO Ying, YUAN Ruo. Electrochemiluminescence Immunosensor Based on Platinum Nanoparticles for the Determination of Apolipoprotein A1[J]. Journal of Electrochemistry, 2016 , 22(3): 299-305.
DOI: 10.13208/j.electrochem.150914
Available at: https://jelectrochem.xmu.edu.cn/journal/vol22/iss3/9