Horseradish Peroxidase/Poly-o- phen ylenediamine Mem brane Electrode:Preparation and Properties

Authors

Dong Wei, Dept.of Chem., State Key Lab. For Phys. Chem.of the Solid Surfaces, Xiamen Univ., Xiamen,361005;
Huihuang Wu, Dept.of Chem., State Key Lab. For Phys. Chem.of the Solid Surfaces, Xiamen Univ., Xiamen,361005;

Abstract

A horseradish peroxidase(HRP)/poly-o-phenylenediamine(PPD)membraneelectrede was prepared by electropolymerization of o-phenylenediamine on glassy carbon in pH 7.0phosphate buffer,the process of the enzyme immobilization was studied,The enzyme electredeobtained exhibits the activity to catalyze the electrored uction of hydrogen peroxide in the absence ofany electron transfer mediator.The red uction reaction occurs in the potential range of PPD redoxprocess,it was believed that the polymer participates in electron transfer of the enzyme.The enzymereaction kinetics at rotating H RP/PPD electrede was analysed,and the factors influencing kineticparameters were discussed.

Keywords

Eezyme electrede, Enzyme electrochemieal immobilization, Horseradishperoxidase, Hydrogen-peroxide reduction, Bioelectrocatalysis

DOI Link

https://doi.org/10.61558/2993-074X.1281

Publication Date

1995-05-28

Online Available Date

1995-05-28

Revised Date

1995-05-28

Received Date

1995-05-28

Recommended Citation

Dong Wei, Huihuang Wu. Horseradish Peroxidase/Poly-o- phen ylenediamine Mem brane Electrode:Preparation and Properties[J]. Journal of Electrochemistry, 1995 , 1(2): 186-192.
DOI: 10.61558/2993-074X.1281
Available at: https://jelectrochem.xmu.edu.cn/journal/vol1/iss2/9

References

1BarlettPN，CcoporJM．Areviewoftheimmobilizationofenzymesinelectropolymerizedfilms．J．Etectroanal．Chem．，1993，362：1￣122ContractorAQ，SureshkumarTN，NarayayanR，SukeerthiS，LalR，SrinivasaRS．Conductingpolymer－basedbiosensors．ElectrochimActa．，1994，39：1321￣13243WollenbergerU，BogelannovskayaV，BobrinS，SchellerF，TarasevichM．Enzymeelectredesusingbioelectroeatalyticreductionofhydrogenperoxide．Anal．Let．，1990，23：1795￣18084TatsumaT，GondairaM，WatanatbeT．Peroxidase－incorporatedpolypyrrolemembraneelectredes．Anal．Chem．，1992，64：1183￣11875BelangerD，NadreauJ，ForterG．Electrochemistryofthepolypyrroleglucoseoxidaseelectrede．J．Electroanal．Chem．．1989，274：143￣1556SunShigang，YangDongfang，TianZhaowu．In－situFTIRspectreecopicstudiesofelectrocatalyticprocesses－oxidationofn－propanolonPtelectrode．ProgressinNaturalScience，1991，1：48￣547ChibaK，OhsakaT，OhhukiY．OyamaN．Electrochemicalprcparationofaladderpolymercontainingphennazinerings．J．Eelctroanal．Chem．，1987．219：117￣1248LeviMD，PisarevskayaFYu．Cyclicandsteady－statevoltammetricstu