Horseradish Peroxidase/Poly-o-phen ylenediamine Mem brane Electrode: Preparation and Properties

Authors

Dong Wei, Dept.of Chem., State Key Lab. For Phys. Chem. of the Solid Surfaces, Xiamen Univ., Xiamen 361005
Huihuang Wu, Dept.of Chem., State Key Lab. For Phys. Chem. of the Solid Surfaces, Xiamen Univ., Xiamen 361005

Corresponding Author

Huihuang Wu

Abstract

A horseradish peroxidase (HRP)/poly-o-phenylenediamine (PPD) membrane electrode was prepared by electropolymerization of o-phenylenediamine on glassy carbon in pH 7.0 phosphate buffer, the process of the enzyme immobilization was studied. The enzyme electrode obtained exhibits the activity to catalyze the electroreduction of hydrogen peroxide in the absence of any electron transfer mediator. The red uction reaction occurs in the potential range of PPD redoxprocess, it was believed that the polymer participates in electron transfer of the enzyme. The enzyme reaction kinetics at rotating HRP/PPD electrode was analysed, and the factors influencing kinetic parameters were discussed.

Keywords

Eezyme electrede, Enzyme electrochemieal immobilization, Horseradishperoxidase, Hydrogen-peroxide reduction, Bioelectrocatalysis

DOI Link

https://doi.org/10.61558/2993-074X.1281

Publication Date

1995-05-28

Online Available Date

1995-05-28

Revised Date

1995-01-24

Received Date

1994-11-24

Recommended Citation

Dong Wei, Huihuang Wu. Horseradish Peroxidase/Poly-o-phen ylenediamine Mem brane Electrode: Preparation and Properties[J]. Journal of Electrochemistry, 1995 , 1(2): 186-192.
DOI: 10.61558/2993-074X.1281
Available at: https://jelectrochem.xmu.edu.cn/journal/vol1/iss2/9