Influence of o-Phenylenediamine on the Catalysis at HRP Enzyme Electrode in 1,4-Dioxane

Authors

Hong Chen, State key Laboratory for Physical Chemistry of the Solid Surface, Departmet of Chemistry, Xiamen University, Xiamen 361005
Hui-huang Wu, State key Laboratory for Physical Chemistry of the Solid Surface, Departmet of Chemistry, Xiamen University, Xiamen 361005

Corresponding Author

Huihuang Wu

Abstract

Horseradish peroxidase (HRP) was immobilized by crosslinkage on the glassycarbon electrode to prepare the enzyme electrode, where the reduction of H₂O₂ was studied in 1,4-dioxane media. It was shown that HRP electrode can catalyze the reduction of H₂O₂ without anymedintor, however, as an activator, o-phenylenediamine can promote the bio-catalysis. In thepresence of the activator, the parallel reactions occurred on the enzyme electrode. The workingcondition of the HRP electrode was investigated as the sensors for H₂O₂ and o-phenylenediamine, also the influence of water content in organic phase was discussed on the performance of the enzyme electrode in organic phase.

Keywords

Horseradish peroxidase, Organic phase enzyme electrode, Activator, Bioelectronic-catalysis

DOI Link

https://doi.org/10.61558/2993-074X.1322

Publication Date

1995-11-28

Online Available Date

1995-11-28

Revised Date

1995-05-05

Received Date

1994-10-21

Recommended Citation

Hong Chen, Hui-huang Wu. Influence of o-Phenylenediamine on the Catalysis at HRP Enzyme Electrode in 1,4-Dioxane[J]. Journal of Electrochemistry, 1995 , 1(4): 465-468.
DOI: 10.61558/2993-074X.1322
Available at: https://jelectrochem.xmu.edu.cn/journal/vol1/iss4/15