Influence of o-Phenylenediamine on the Catalysis at HRP Enzyme Electrode in l,4-Dioxane

Authors

Hong Chen, State key Laboratory for Physical cyhemistry of the Solid Surface,Departmet of Chemistry, Xiamen University,Xiamen,Fujian 361005;
Hui-huang Wu, State key Laboratory for Physical cyhemistry of the Solid Surface,Departmet of Chemistry, Xiamen University,Xiamen,Fujian 361005;

Abstract

Horseradish peroxidase(HRP)was immobilized by crosslinkage on the glassycarbon electrode to prepare the enzyme electrede ,where the reduction of H_2O_2 was studied in l,4-dioxane media. It was shown that HRP electrode can catalyze the reduction of H_2O_2 without anymedintor,however, as an activator,o-phenylenediamine can promote the bio-catalysis. In thepresence of the activator,the parallel reactions occurred on the enzyme electrode. The workingcondition of the HRP electrede was investigated as the sensors for H_2O_2 and o-phenylenediamine,alsothe influence of water content in organic phase was discussed on the performance of the enzymeelectrode in organic phase.

Keywords

Horseradish peroxidase, Organic phase enzyme electrode, Activator, Bioelectronic-catalysis

DOI Link

https://doi.org/10.61558/2993-074X.1322

Publication Date

1995-11-28

Online Available Date

1995-11-28

Revised Date

1995-11-28

Received Date

1995-11-28

Recommended Citation

Hong Chen, Hui-huang Wu. Influence of o-Phenylenediamine on the Catalysis at HRP Enzyme Electrode in l,4-Dioxane[J]. Journal of Electrochemistry, 1995 , 1(4): 465-468.
DOI: 10.61558/2993-074X.1322
Available at: https://jelectrochem.xmu.edu.cn/journal/vol1/iss4/15