Electrochemical Behaviours of Hemoglobin-DDAB-Modified Powder Microelectrode and the Application of the Powder Microelectrode in Determination of Nitric Oxide

Authors

Zhi-mou GUO, College of Chemistry and Molecular Science, Wuhan University, Wuhan 430072,China;
Huan LIU, College of Chemistry and Molecular Science, Wuhan University, Wuhan 430072,China;
Jian CHEN, College of Chemistry and Molecular Science, Wuhan University, Wuhan 430072,China;
Wen-ting ZHANG, College of Chemistry and Molecular Science, Wuhan University, Wuhan 430072,China;

Corresponding Author

Jian CHEN(chenjian@dicp.ac.cn)

Abstract

The electrochemical behaviours of DDAB-Hb-PME were studied in this paper. The (apparent) surface concentration of Hb was enhanced greatly by using the powder microelectrode (technique). NO reacts with Hb and absorbs on the surface of the modified carbon powder. Therefore, the output of the electrode was enhanced greatly by employing PME with higher reaction surface (area). The sensitivity of DDAB-Hb-PME is 3.1 mA/μmol·L~(-1)·cm~(2), which is much larger than the values obtained with the traditional electrodes. The detection limit is 9 nmol·L~(-1) and the linear response range is 9～100 nmol·L~(-1).

Keywords

Hemoglobin, Powder microelectrode, Nitric oxide

DOI Link

https://doi.org/10.61558/2993-074X.1593

Publication Date

2004-11-28

Online Available Date

2004-11-28

Revised Date

2004-11-28

Received Date

2004-11-28

Recommended Citation

Zhi-mou GUO, Huan LIU, Jian CHEN, Wen-ting ZHANG. Electrochemical Behaviours of Hemoglobin-DDAB-Modified Powder Microelectrode and the Application of the Powder Microelectrode in Determination of Nitric Oxide[J]. Journal of Electrochemistry, 2004 , 10(4): 404-408.
DOI: 10.61558/2993-074X.1593
Available at: https://jelectrochem.xmu.edu.cn/journal/vol10/iss4/6

References

[1]　RuslingJF.Enzymebioelectrochemistryincastbiomembrane likefilms[J].Acc.Chem.Res.,1998,31:363.
[2]　ZhangHL,YuA,ChenHY,Electrocatalyticreductionofnitricoxideonmyoglobinmodifiedelectrode[J].ActaChimicaSinica,1997,55:486.
[3]　MimicaD,ZagalJH,BediouiF.Electroreductionofnitritebyhemin,myoglobinandhemoglobininsurfactantfilms[J].J.Electroanal.Chem.,2001,497:106.
[4]　ChenJ,ChaCS.Detectionofdopamineinthepresenceofalargeexcessofascorbicacidbyusingthepowdermicroelectrodetechnique[J].J.Electroanal.Chem.,1999,463:93.
[5]　ChaCS,LiCM,YangHX,etal,Powdermicroelectrodes[J].J.Electroanal.Chem.,1994,368:47.
[6]　HuangH,HuNF,ZengYH,etal.Electrochemistryandelectrocatalysiswithhemeproteinsinchitosanbiopolymerfilms[J].AnalyticalBiochemistry,2002,308:141.
[7]　LiuHY,WangLW,HuNF.Directelectrochemistryofhemoglobininbiomembrane likeDHP PDDApolyion surfactantcompositefilms[J].ElectrochimicaActa,2002,47:2515.
[8]　HanXJ,HuangWM,JiaJB,etal,Directelectrochemistryofhemoglobininegg phosphatidylcholinefilmsanditscatalysistoH2O2[J].Biosensors＆Bioelectronics,2002,17:741.
[9]　FanCH,LiGX,ZhuJQ,etal.AreagentlessnitricoxidebiosensorbasedonhemoglobinDNAfilms[J].AnalyticaChimicaActa,2000,423:95.
[10]　WuFH,ZhaoGC,WeiXW,Electrocatalyticoxidationofnitricoxideatmulti walledcarbonnanotubesmodifedelectrode[J].ElectrochemistryCommunications,2002,4:690.
[11]　PalmeriniCarloA,ArientiGiuseppe,PalombariRoberto.Electrochemicaldeterminationofnitricoxideandofitsderivatives[J].Talanta,2003,61:37.