The electrochemical impedance spectrum(EIS) of ferrocene(Fc) at a rotating glassy carbon(GC) electrode and its interaction with calf thymus DNA in Tris-NaCl buffer solution(pH=7.2) have been investigated by using electrochemical techniques such as rotating disk electrode(RDE),EIS and its data fitting.A plateau of the limiting current for the voltammetric curve and two capacitance arches obtained at the rotating GC electrode are exhibited.The interaction of Fc with DNA is represented by the decrease of limiting diffusion current and the increase of electrochemical reaction resistances with the increase of DNA concentration,respectively.On the other hand,based on the theoretical analysis and data fitting of RDE and EIS,values of the rate constant at the formal potential obtained by RDE are consistent with those by EIS on the rotating GC electrode.At the same time,the rate constant at the formal potential decreases in the presence of DNA.


Ferrocene, DNA, Rotating disk electrode, Electrochemical impedance spectrum

Publication Date


Online Available Date


Revised Date


Received Date



[1]S ilvestri A,Barone G,Ru isi G.,et al.The interactionof native DNA w ith iron(III)-N,N′-ethylene-b is(salicyli-dene im inato)-chloride[J].J.Inorg.B iochem.,2004,98(4):589~594.
[2]Jiang Cai-wu(蒋才武).Synthesis,characterization andDNA b ind ing stud ies of novel asymm etric tridentate poly-pyrid ine ligand and the ir heteroleptic ruthen ium(Ⅱ)comp lexes[J].Acta Ch im.S in ica,2004,62(7):692~696.
[3]Zhou Y L,L i Y Z.Stud ies of the interaction between po-ly(d iallyld im ethyl ammon ium chloride)and DNA byspectroscop ic m ethods[J].Colloids and Surfaces A:Physicochem.Eng.Aspects,2004,233:129~135.
[4]Yang Gong-jun(杨功俊),Xu Jing-juan(徐静娟),ChenHong-yuan(陈洪渊).Stud ies on the interaction betweencatecholam ine derivatives and DNA by m eans of spectro-scop ic and voltamm etric m ethods[J].Chem.J.Ch ineseUn iversities,2004,25(7):1235~1239.
[5]W ang Shu-ling(王树玲),Yu Jun-sheng(于俊生).SERS stud ies on the interaction of berberine w ith ctDNA[J].Chem.J.Ch inese Un iversities,2002,23(9):1676~1679.
[6]Palan isamy U M,M allayan P.DNA b ind ing and c leavageactivity of[Ru(NH3)4(d iim ine)]C l2comp lexes[J].Inorg.Ch im.Acta,2004,357:901~912.
[7]Jiang C W.Ch iral d iruthen ium(II)comp lexes contain inga mod ified 4,4′-b ipyrid ine bridging ligand:syntheses,characterization and DNA b ind ing[J].Inorg.Ch im.Ac-ta,2004,357:3403~3406.
[8]Fazlu lH,Jun Q Y,Hassan D,et al.Stud ies on activi-ties,cell uptake and DNA b ind ing of fourtrans-p lanara-m inep latinum(II)comp lexes of the form:trans-PtL(NH3)C l2,where L=2-hydroxypyrid ine,im idazole,3-hydroxypyrid ine and im idazo(1,2-α)pyrid ine[J].J.Inorg.B iochem.,2004,98:1261~1270.
[9]Pérez-CabréM,Cervantes G,Moreno V,et al.Pd(II)and Pt(II)comp lexes w ith arom atic d iam ines:study ofthe ir interaction w ith DNA[J].J.Inorg.B iochem.,2004,98:510~521.
[10]Ibrah im M S,Shehatta I S,A l-Nayeli A A.Voltamm et-ric stud ies of the interaction of lum azine w ith cyc lodex-trins and DNA[J].J.Pharm.B iom ed.Anal.,2002,28:217~225.
[11]Tang P ing(唐平),Zeng Bai-zhao(曾百肇).E lecro-chem ical behavior of ep inephrine at deoxyribonuc le ic aidmod ified gold electrodes and influence of lead iron[J].Ch inese J.Anal.Chem.,2003,31(6):641~645.
[12]Zhou Jian-zhang(周剑章),W u L ing-ling(吴玲玲),Dong L i-q in(董丽琴),et al.Immob ilization of longstrand DNA onto gold surface and electrochem ical labe-ling of hybrid ization ofDNA[J].E lectrochem.,2001,7(3):276~280.
[13]LüJia-gen(吕家根),Zhang Zhu-jun(章竹君),ZhengHu-zh i(郑鹄志).In vivo determ ination of copper m e-tabolism in rabb it serum based on a new electrochem ilu-m inescence[J].Acta Ch im.S in ica,2002,60(7):1274~1278.
[14]Pang D W,Abr a H D.M icrom ethod for the investiga-tion of the interactions between DNA and redox-activemolecu les[J].J.Anal.Chem.,1998,70(15):3162~3169.
[15]L i Hong(李红),Jiang X iong(蒋雄),W ang Le i(王雷),et al.E lectrochem ical stud ies on the interactionsof the comp lex of Co(phen)2TATP3+w ith DNA at rota-ting Au-Au electrode[J].Chem.J.Ch inese Un iversi-ties,2000,212(7):995~998.
[16]B rett C M A,O live ira B A M,Serrano S H P.An EISstudy ofDNA-mod ified electrodes[J].E lectroch im.Ac-ta,1999,44:4233~4239.
[17]Popova L V,Bab in V N,Belousov Y A,et al.Antitu-mor effects of b inuc lear ferrocene derivatives[J].App l.O rganom et.Chem.,1993,7:85~94.
[18]CarterM T,RodriguezM,Bard A J.Voltamm etric stud-ies of interaction of m etal chelates w ith DNA.2.Tris-chelated comp lexes of cobalt(Ⅲ)and iron(Ⅱ)w ith 1,10-phenanthroline and 2,2-′b ipyrid ine[J].J.Am.Chem.Soc.,1989,111(24):8901~8911.
[19]Bard A J,Fau lkner L R.E lectrochem icalM ethods:Fun-dam entals and App lication[M],New York:JohnW ileyand Sons,1980.92~292.
[20]Randriam ahazaka H,Noёl V,Chevrot C.Fractal d i-m ension of the active zone for a p-doped poly(3,4-ethyl-ened ioxyth iophene)mod ified electrode towards a ferro-cene probe[J].J.E lectroanal.Chem.,2003,556:35~42.



To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.