Immobilization of Cytochrome c on the Surface of Single-wall Carbon Nanotube and Its Direct Electron Transfer and Electrocatalysis

Authors

Ya-jing YIN, Department of Chemistry,Jiangsu Key Laboratory for Molcular and Medical Biotechnology,Nanjing Normal University,Nanjing 210097,Jiangsu,China;
Ya-fen LV, Department of Chemistry,Jiangsu Key Laboratory for Molcular and Medical Biotechnology,Nanjing Normal University,Nanjing 210097,Jiangsu,China;
Ping WU, Department of Chemistry,Jiangsu Key Laboratory for Molcular and Medical Biotechnology,Nanjing Normal University,Nanjing 210097,Jiangsu,China;
Pan DU, Department of Chemistry,Jiangsu Key Laboratory for Molcular and Medical Biotechnology,Nanjing Normal University,Nanjing 210097,Jiangsu,China;
Yan-mao SHI, Department of Chemistry,Jiangsu Key Laboratory for Molcular and Medical Biotechnology,Nanjing Normal University,Nanjing 210097,Jiangsu,China;
Chen-xin CAI, Department of Chemistry,Jiangsu Key Laboratory for Molcular and Medical Biotechnology,Nanjing Normal University,Nanjing 210097,Jiangsu,China;

Abstract

Cytochrome c(Cyto c) was immobilized on the surface of the single-wall carbon nanotube(SWNT) by using the method of adsorption.Infrared spectroscopy indicated that the Cyto c remained in its original structure and did not undergo structural change after its immobilization on the SWNT.The direct electrochemistry of cyto c,which was adsorbed on the surface of the SWNT,was studied by cyclic voltammetry.The voltammetric results demonstrated that the SWNT had promoting effects on the direct electron transfer of Cyto c and also indicated that the immobilized Cyto c retained its bioelectrocatalytic activity to the reduction of H_(2)O_(2).This modified electrode might be used in development of new biosensors and the biofuel cells.

Keywords

Carbon nanotube, Chemically modified electrode, Direct electrochemistry, Cytochrome c

DOI Link

https://doi.org/10.61558/2993-074X.1741

Publication Date

2006-08-28

Online Available Date

2006-08-28

Revised Date

2006-08-28

Received Date

2006-08-28

Recommended Citation

Ya-jing YIN, Ya-fen LV, Ping WU, Pan DU, Yan-mao SHI, Chen-xin CAI. Immobilization of Cytochrome c on the Surface of Single-wall Carbon Nanotube and Its Direct Electron Transfer and Electrocatalysis[J]. Journal of Electrochemistry, 2006 , 12(3): 298-303.
DOI: 10.61558/2993-074X.1741
Available at: https://jelectrochem.xmu.edu.cn/journal/vol12/iss3/13

References

[1]Yu J H,L iu S Q,Ju H X.M ed iator-free phenol sensorbased on titan ia sol-gel encapsu lation m atrix for immob i-lization of tyrosinase by a vapor deposition m ethod[J].B iosen.B ioelectron,2003,19:509~514.
[2]Barton S C,Gallaway J,Atanassov P.Enzym atic b iofu-el cells for imp lantab le and m icroscale devices[J].Chem.Rev.,2004,104:4867~4886.
[3]Cai Chen-xin(蔡称心),Chen Jing(陈静),Lu Tian-hong(陆天虹).D irect electron transfer of glucose oxi-dase at carbon nanotube mod ified electrode[J].Sc i.inCh ina(Ser.B),2003,33:511~518.
[4]Sun Dong-m e i(孙冬梅),Cai Chen-xin(蔡称心),X ingW e i(邢巍),et al.Immob ilization and d irect e-lectrochem istry of Cu-contained oxidase on the surface ofactive carbon powder[J].Ch inese Sc i.Bu ll.,2004,17:1722~1724.
[5]Hess C R,Juda G A,Dooley D M,et al.Gold elec-trodes w ired for coup ling w ith the deep ly buried activesite ofArthrobacter globiform isam ine oxidase[J].J.Am.Chem.Soc.,2003,125:7156~7157.
[6]Cai C X,Chen J.D irect electron transfer and b ioelec-trocatalysis of hemoglob in at a carbon nanotube electrode[J].Anal.B iochem.,2004,325:285~292.
[7]Aguey-Z insou K F,Bernhardt P V,Kapp ler U,et al.D irect electrochem istry of a bacterial su lfite dehydrogen-ase[J].J.Am.Chem.Soc.,2003,125:530~535.
[8]Dong Shao-jun(董绍俊),Che Guang-li(车广礼),X ie Yuan-wu(谢远武).Chem ically Mod ified E lec-trode[M].2nd.Be ijing:Sc ience Press,2003,456~570.
[9]V incent K A,Arm strong F A.Investigating m et-alloenzym e reaction using electrochem ical sweeps andsteps:F ine control and m easurem ents w ith reactantsranging from ions to gases[J].Inorg.Chem.,2005,44:798~809.
[10]Léger C,E lliott S J,Hoke K R,et al.Enzym e electo-k inetics:using prote in film voltamm etry to investigateredox enzym e and the ir m echan ism[J].B iochem.,2003,42:8653~8662.
[11]Shumyantseva V V,Ivanov Y D,B istolas N,et al.D irect electron transfer of cytochrom e P450 2B4 at e-lectrodes mod ified w ith non ion ic detergent and colloidalc lay nanopartic les[J].Anal.Chem.,2004,76:6046~6052.
[12]Fantuzzi A,Fairhead M,G ilard G.D irect electro-chem istry of immob ilized hum an cytochrom e P450 2E1[J].J.Am.Chem.Soc.,2004,126:5040~5041.
[13]Cai Chen-xin(蔡称心),Chen Jing(陈静).D irectelectron transfer of redox prote ins and enzym es promo-ted by carbon nanotube[J].E lectrochem istry(in Ch i-nese),2004,10(2):159~167.
[14]Cai C X.The d irect electrochem istry of cytochrom ecat a gold m icroband electrode mod ified w ith 4,6-d im e-thyl-2-m ercaptopyrim id ine[J].J.E lectroanal.Chem.,1995,393:119~122.
[15]W ang J X,L i M X,Sh i Z J,et al.D irect electro-chem istry of cytochrom ecat a glassy electrode mod i-fied w ith single-wall carbon nanotubes[J].Anal.Chem.,2002,74:1933~1997.
[16]Cheng F L,Du S,Jin B K.E lectrochem ical stud ies ofcytochrom econ electrodes mod ified by single-wall car-bon nanotubes[J].Ch in.J.Chem.,2003,21:436~441.
[17]Lojou,B ianco P.M embrane electrodes can modu latethe electrochem ical response of redox prote ins-d irect e-lectrochem istry of cytochrom ec[J].J.E lectroanal.Chem.,2000,485:71~80.
[18]Lojou,G iud ic i-O rticon iM T,B ianco P.D irect elec-trochem istry and enzym atic activity of bacterial poly-hem ic cytochrom ec3incorporated in c lay film s[J].J.E lectroanal.Chem.,2005,579:199~213.
[19]W ang J,Musam eh M,L in Y H.Solub ilization of car-bon nanotubes by nafion toward the preparation of am-perom etric b iosensors[J].J.Am.Chem.Soc.,2003,125:2408~2409.
[20]Chen J,Bao J,Cai C X,et al.E lectrocatalytic oxida-tion ofNADH at an ordered carbon nanotubes mod ifiedglassy carbon electrode[J].Anal.Ch im.Acta,2004,516:29~34.
[21]Chen J,Cai C X.D irect electrochem ical oxidation ofNADPH at a low potential on the carbon nanotube mod-ified glassy carbon electrode[J].Ch in.J.Chem.,2004,22:167~171.
[22]Musam eh M,W ang J,M erkoc i A,et al.Low-poten-tial stab le NADH detection at carbon-nanotube-mod i-fied glassy carbon electrodes[J].E lectrochem.Com-mun.,2002,4:743~746.
[23]B ritto P J,Santhanam K S V,A jayan P M.Carbonnanotube electrode for oxidation of dopam ine[J].B io-electrochem.B ioenerg.,1996,41:121~125.
[24]Chen J,Bao J,Cai C X.Fabrication,characterizationand electro catalysis of an ordered carbon nanotube e-lectrode[J].Ch in.J.Chem.,2003,21:665~669.
[25]W angM,Shen Y,L iu Y,et al.D irect electrochem is-try of m icroperxidase 11 using carbon nanotube mod i-fied electrodes[J].J.E lectroanal.Chem.,2005,578:121~127.
[26]Yan Y,ZhengW,Zhang M,et al.B ioelectrochem is-try functional nanohybrids through co-assemb ling ofprote ins and surfactants onto carbon nanotubes:fac ili-tated electron transfer of assemb led prote ins w ith en-hanced farad ic response[J].Langmu ir,2005,21:6560~6566.
[27]Cai Chen-xin(蔡称心),Chen Jing(陈静).D irectelectrochem istry of horserad ish peroxidase at a carbon-nanotube electrode[J].Acta Ch im ica S in ica,2004,62:335~340.
[28]Patolsky F,W e izm ann Y,W illner I.Long-range elec-trical contacting of redox enzym es by SWCNT connec-tors[J].Angew.Chem.Int.Ed.,2004,43:2113~2117.
[29]Cai Chen-xin(蔡称心),Chen Jing(陈静),BaoJian-chun(包建春),et al.A llications of carbonnanotubes in analytical chem istry[J].Ch inese J.A-nal.Chem.,2004,32:381~387.
[30]Sun D M,Cai C X,L i X G,et al.D irect electro-chem istry and b ioelectrocatalysis of horserad ish peroxi-dase immob ilized on active carbon[J].J.E lectroanal.Chem.,2004,566:415~421.
[31]L i Y M,L iu H H,Pang D W.D irect electrochem istryand catalysis of hem e-prote ins entrapped in m ethyl cel-lu lose film s[J].J.E lectroanal.Chem.,2004,574:23~31.
[32]Zhao G C,Y in Z Z,Zhang L,et al.D irect electro-chem istry of cytochrom econ a mu lti-walled carbonnanotubes mod ified electrode and its electrocatalyticactivity for the reduction of H2O2[J].E lectrochem.Commun.,2005,7:256~260.