Interfacial Electrochemical Behavior of Fibrinogen on Pt Electrode

Authors

Song-mei CHEN, Key Lab.of advanced Technology of Materials of Education Ministry of China,Collge of Materials Science and Engineering,Southwest Jiaotong University,Chengdu 610031,China;State Key Lab.of Physical Chemistry of the Solid Surface,Dept.of Chemistry,Xiamen University,Xiamen 361005,Fujian,China;
Jian-zhang ZHOU, State Key Lab.of Physical Chemistry of the Solid Surface,Dept.of Chemistry,Xiamen University,Xiamen 361005,Fujian,China;
Zhong-hua LIN, State Key Lab.of Physical Chemistry of the Solid Surface,Dept.of Chemistry,Xiamen University,Xiamen 361005,Fujian,China;
Dong-mei ZENG, State Key Lab.of Physical Chemistry of the Solid Surface,Dept.of Chemistry,Xiamen University,Xiamen 361005,Fujian,China;
Nan HUANG, Key Lab.of advanced Technology of Materials of Education Ministry of China,Collge of Materials Science and Engineering,Southwest Jiaotong University,Chengdu 610031,China;
Guo-jiang WAN, Key Lab.of advanced Technology of Materials of Education Ministry of China,Collge of Materials Science and Engineering,Southwest Jiaotong University,Chengdu 610031,China;State Key Lab.of Physical Chemistry of the Solid Surface,Dept.of Chemistry,Xiamen University,Xiamen 361005,Fujian,China;

Abstract

The interfacial electrochemical behaviors of fibrinogen on Pt electrode in phosphate buffered solution(PBS) was investigated by using cyclic voltammetry and electrochemical in-situ FTIR spectroscopy.The cyclic voltammetry results demonstrated that the fibrinogen adsorb on Pt electrode and impede the interfacial charge transfer of hydrogen ion reaction process as well as of oxygen reduction process,which is increased with higher scan rate.The cyclic voltammetry spectra of platinum absorbed with fibrinogen in K3[Fe(CN)6] and K4[Fe(CN)6]·3H2O solutions shows that the fibrinogen can partly decrease the oxidation and reduction current peaks.There is no specific anodic and cathodic current peaks found to attributed from fibrinogen within potential scanned rang of-0.1~0.6V(vs.SCE).Nevertheless,electrochemical in-situ FTIR spectra indicate the fibrinogen undergo chemical reaction when which anodic potential higher than 0.3~0.5V(vs.SCE).

Keywords

fibrinogen, electrochemical in-situ FTIRS, interfacial electrochemical behavior, coagulation

DOI Link

https://doi.org/10.61558/2993-074X.1863

Publication Date

2008-02-28

Online Available Date

2008-02-28

Revised Date

2008-02-28

Received Date

2008-02-28

Recommended Citation

Song-mei CHEN, Jian-zhang ZHOU, Zhong-hua LIN, Dong-mei ZENG, Nan HUANG, Guo-jiang WAN. Interfacial Electrochemical Behavior of Fibrinogen on Pt Electrode[J]. Journal of Electrochemistry, 2008 , 14(1): 56-60.
DOI: 10.61558/2993-074X.1863
Available at: https://jelectrochem.xmu.edu.cn/journal/vol14/iss1/12

References

[1]Cheng Qing(程青),Wang Guo-bin(王国斌).Bloodmaterial interaction[J].ShangHai Biomedical Engi-neering,2001,2(3):33.
[2]Peiqing,Ana S Viana,Luisa M Abrantes,et al.Ado-sorpotion of human serum albumin onto gold:a com-bined electrochemical and ellipsometric study[J].Jour-nal of Colloid and Interfaces Science,2004,279:95.
[3]Sibel Tunc,Manfred F Matiz,Gerald Steiner,et al.Insitu conformational analysis of fibrinogen adsorbed on Sisurfaces[J].Colloids and Surfaces B,2005,42:219.
[4]Robert T TGettens,Jeremy L Gilbert.Quantification offibrinogen adsorption onto 316L stainless steel[J].Journal of Biomedical Materials,2006,11:465.
[5]Ramasamyl N,Ranganathan M,Duic L,et al.Electro-chemical behavior of blood coagulation factors[J].JElectrochem Soc,1973,120:354.
[6]Douglas R Jackson,Sasha Omanovic,Sharon G Ros-coe.Electrochemical studies of the assorption behaviorof serum proteins on titanium[J].Langmiur,2000,16:5449.
[7]Wang Jie,Chen Xiao-yun,Mattew L Clarke,et al.Vibrational spectroscopic studies on fibrinogen adsorp-tion at polystyrene/protein solution interfaces:Hydro-phobic side chain and secondary structure changes[J].J Phys Chem B,2006,110:5017.
[8]MatthewLClarke,Wang Jie,Chen Zhan.Conformation-al changes of fibrinogen after adsorption[J].J PhysChem B,2005,109(46):22027.
[9]Sawyer P N.Significance of electrochemical phenomenain intravascular thrombosis[J].Nature,1965,206(2):1162.
[10]Sun Shi-gang,Gong Hui.Research methods of solidcatalyst[J].Petrochemical Industry,2001,30(10):806.
[11]Luo Guo-an(罗国安),Wang Zong-hua(王宗花),Wang Yi-ming(王义明).Applications and fabricationof the bio-compatible electrode[M].Beijing:SciencePublishing House,2006.286.
[12]Patrick Vermette,Virginie Gauvreau,Michel Pezolet,et al.Albumin and fibrinogen adsorption onto phos-phatidylcholine monolayers investigated by fouriertransform infrared[J].Colloids and Surfaces B,2003,29:285.
[13]Xing Xu-ying(邢徐颖),Chen Shi-di(陈世娣),ShenSi-yun(沈思云).The use guide of infrared spectra[M].Beijing:Science Publishing House,1992.198.
[14]Stamatin L,Cristescu R,Socol G,et al.Laser deposi-ton of fibrinogen blood proteins thin films by matrix as-sisted pulsed laser evaporation[J].Applied SurfaceScience,2005,248:422.