Study on the Binding mode of In(bpy)Cl_3·H_2O with DNA by the Methods of Electrochemistry

Authors

Jun-wei WANG, Institute of Molecular Science,Key Laboratory of Chemical Biology andMolecular Engineering of Ministry of Education,Shanxi University,Taiyuan 030006,China;
Jian-guo REN, Institute of Molecular Science,Key Laboratory of Chemical Biology andMolecular Engineering of Ministry of Education,Shanxi University,Taiyuan 030006,China;
Bao-juan ZHOU, Institute of Molecular Science,Key Laboratory of Chemical Biology andMolecular Engineering of Ministry of Education,Shanxi University,Taiyuan 030006,China;
Xiao-ling GAO, Institute of Molecular Science,Key Laboratory of Chemical Biology andMolecular Engineering of Ministry of Education,Shanxi University,Taiyuan 030006,China;
Yan-ni TIAN, Institute of Molecular Science,Key Laboratory of Chemical Biology andMolecular Engineering of Ministry of Education,Shanxi University,Taiyuan 030006,China;

Abstract

The complex In(bpy)Cl3·H2O was synthesized.The binding mode of the complex with DNA was studied by the methods of cyclic voltammetry(CV),differential pulse voltammetry and ac impedance in the buffer Tris-HCl(pH=7.2).A quasi-reversible redox wave for the central Indium ion from the cyclic voltammograms was shown at a PG electrode.It has been found that after the complex acted with DNA,The complex's formal potential negatively shifted from-0.696 V to-0.717 V,the values of the reduction peak current and apparent diffusion coefficient decreased significantly.The results of differential pulse voltammetry were the same as cyclic voltammetry,and the electrochemical reaction resistance values increased from 143 Ω·cm2 to 169 Ω·cm2.The result indicated that the complex In(bpy)Cl3·H2O acted with DNA apparently.The binding mode of the complex with DNA was stable-electricity binding also medium degree exterior bonding mode was manifested.

Keywords

In(bpy)Cl3·H2O, calf thymus DNA, stable-electricity binding

DOI Link

https://doi.org/10.61558/2993-074X.1866

Publication Date

2008-02-28

Online Available Date

2008-02-28

Revised Date

2008-02-28

Received Date

2008-02-28

Recommended Citation

Jun-wei WANG, Jian-guo REN, Bao-juan ZHOU, Xiao-ling GAO, Yan-ni TIAN. Study on the Binding mode of In(bpy)Cl_3·H_2O with DNA by the Methods of Electrochemistry[J]. Journal of Electrochemistry, 2008 , 14(1): 71-75.
DOI: 10.61558/2993-074X.1866
Available at: https://jelectrochem.xmu.edu.cn/journal/vol14/iss1/15