Direct Electrochemistry of Chloroperoxidase at GC Electrode Modified with SWNTs

Authors

Hong-xia ZHANG, Department of Chemistry,Shanghai Normal University,Shanghai 200234,China;
Xia-qin WU, Department of Chemistry,Shanghai Normal University,Shanghai 200234,China;
Wen-jing HOU, Department of Chemistry,Shanghai Normal University,Shanghai 200234,China;
Wen XIE, Department of Chemistry,Shanghai Normal University,Shanghai 200234,China;
Zhong-qing LU, Department of Chemistry,Shanghai Normal University,Shanghai 200234,China;
Rong WANG, Department of Chemistry,Shanghai Normal University,Shanghai 200234,China;
He-xing LI, Department of Chemistry,Shanghai Normal University,Shanghai 200234,China;

Corresponding Author

Xia-qin WU

Abstract

The chloroperoxidase(CPO) was immobilized onto the surface of SWNTs modified electrodes.The electrochemical behaviors of modified electrode have been investigated by cyclic voltammetry.The appearance of a pair of well-defined redox peaks in the CV curve indicated that SWNTs can promote the electron transfer reaction of CPO.The results of cyclic voltammetry in PBS of different pH,from 3~9,showed that the reaction of CPO on the modified electrode was with equal numbers of electron and protein.This readily prepared SWNTs modified electrode showed excellent stability and good catalysis to O2.

Keywords

chloroperoxidase, carbon nanotubes, direct electrochemistry, modified electrode, oxygen reduction, electrocatalysis

DOI Link

https://doi.org/10.61558/2993-074X.1926

Publication Date

2008-11-28

Online Available Date

2008-11-28

Revised Date

2008-11-28

Received Date

2008-11-28

Recommended Citation

Hong-xia ZHANG, Xia-qin WU, Wen-jing HOU, Wen XIE, Zhong-qing LU, Rong WANG, He-xing LI. Direct Electrochemistry of Chloroperoxidase at GC Electrode Modified with SWNTs[J]. Journal of Electrochemistry, 2008 , 14(4): 383-387.
DOI: 10.61558/2993-074X.1926
Available at: https://jelectrochem.xmu.edu.cn/journal/vol14/iss4/7

References

[1]Lowell P Hager,David R Morris,Frederick S Brown,etal.Chloroperoxidase II.Utilization of halogen anions[J].Biological Chem,1966,241(8):1769-1777.
[2]Fang Wei-shuo(方唯硕),Cheng Ke-di(程克棣).Application of peroxidase in asymmetric oxidation andthe reaction mechanism[J].Organic Chemistry(inChinese),2002,22(10):710-717.
[3]Lu Zhong-qing,Hou Wen-jing,Wu Xia-qin,et al.E-lectrochemistry of self-assembled ultra-thin films com-posed of chloroperoxidase and polyions[J].ChemLett,2007,36(4):564-565.
[4]Kalayil M Manesh,Santhosh P,Anantha I Gopalan,etal.Electrocatalytic dioxygen reduction at glassy carbonelectrode modified with polyaniline grafted multiwall car-bon nanotube film[J].Electroanalysis,2006,18(16):1564-1571.
[5]Zhao Li-yun,Liu Hong-yun,Hu Nai-fei.Assembly oflayer-by-layer films of heme proteins and single-walledcarbon nanotubes electrochemistry and electrocatalysis[J].Anal Bioanal Chem,2006,384:414-422.
[6]L櫣Ya-fen,Yin Ya-jing,Wu Ping,et al.Direct elec-trochemistry and bioelectrocatalysis of myoglobin at acarbon nanotube-modified electrode[J].Acta Phys-Chim Sin,2007,23(1):5-11.
[7]Gong Kuanping,Yu Dong,Xiong Shaoxiang,et al.No-vel electrochemical method for sensitive determination ofhomocysteine with carbon nanotube-based electrodes[J].Biosensors and Bioelectronics,2004,20:253-259.
[8]Mai Zhi-bin(麦智彬),Tan Xue-cai(谭学才),ZouXiao-yong(邹小勇).Progresses of the chemical modi-fied electrodes and electrochemical biosensors based oncarbon nanotubes[J].Journal of Instrumental Analysis(in Chinese),2006,25(3):120-125.
[9]Huang He,Hu Nai-fei,Zeng Yong-huai,et al.Electro-chemistry and electrocatalysis with heme proteins in chi-tosan biopolymer films[J].Analytical Biochemistry,2002,308(1):141-151.