Abstract
Non-specific absorption of enzyme and nucleotide is a main source leading to the background staining in enzyme-amplified amperometric detection of DNA. To eliminate the background staining in this system, the blocking procedure by mercapto-hexanol ( MCH) and bovine serum albumin ( BSA) was studied systematically. The results show that the anti-fouling effect of BSA is better than that of MCH. A simple and efficient blocking strategy employing BSA as the sole blocking reagent was established and applied in the detection of PMLRARα fusion gene in acute promyelocytic leukemia. It can effectively eliminate the background staining,shorten the pre-processing time,and achieve strong signal amplification.
Keywords
electrochemical DNA sensor, enzyme-amplified amperometric assay, bovine serum albumin, blocking
Publication Date
2010-05-28
Online Available Date
2010-05-28
Revised Date
2010-05-28
Received Date
2010-05-28
Recommended Citation
Miao HE, Wei CHEN, Xiong-wei XU, Ai-lin LIU, Xin-hua LIN.
Blocking Method in Enzyme-Amplified Amperometric DNA Biosensor[J]. Journal of Electrochemistry,
2010
,
16(2): Article 21.
DOI: 10.61558/2993-074X.3349
Available at:
https://jelectrochem.xmu.edu.cn/journal/vol16/iss2/21
