Abstract
The electrochemical behavior of kaempferide at activated GCE and the interaction of kaempferide with human serum albumin( HSA) were investigated by cyclic voltammetry. A differential pulse voltammetric method has been proposed for the determination of the HSA with kaempferide. In pH 4. 5 phosphate buffer solutions ( PBS) kaempferide can react with HSA to form an electrochemically non-active supermolecular complex at activated GCE,resulting in the decrease of oxidation peak ( at 0. 468 V) without the change of peak potential. The decrease of the peak current is proportional to HSA concentration in the range of 0. 05 ～ 0. 25 mg/L. The limit of the detection is 0. 008 mg/L. This method can be applied to the determination of HSA sample.
Keywords
kaempferide, protein, voltammetry
Publication Date
2010-05-28
Online Available Date
2010-05-28
Revised Date
2010-05-28
Received Date
2010-05-28
Recommended Citation
Li-qing LIN, Xin-hua LIN, Jing-hua CHEN, Li-ying HUANG, Yin-huan LIU, Guang-wen LI.
Electrochemical Behavior of Kaempferide Interaction with Protein[J]. Journal of Electrochemistry,
2010
,
16(2): Article 22.
DOI: 10.61558/2993-074X.3350
Available at:
https://jelectrochem.xmu.edu.cn/journal/vol16/iss2/22
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