Electrochemical Behavior of Kaempferide Interaction with Protein

Authors

• Li-qing LIN, Department of Pharmaceutical Analysis,Fujian Medical University, Fuzhou 350004, China;
• Xin-hua LIN, Department of Pharmaceutical Analysis,Fujian Medical University, Fuzhou 350004, China;Follow
• Jing-hua CHEN, Department of Pharmaceutical Analysis,Fujian Medical University, Fuzhou 350004, China;
• Li-ying HUANG, Department of Pharmaceutical Analysis,Fujian Medical University, Fuzhou 350004, China;
• Yin-huan LIU, Department of Pharmaceutical Analysis,Fujian Medical University, Fuzhou 350004, China;
• Guang-wen LI, Department of Pharmaceutical Analysis,Fujian Medical University, Fuzhou 350004, China;

Corresponding Author

Xin-hua Lin(xinhua63@163.com)

Abstract

The electrochemical behavior of kaempferide at activated GCE and the interaction of kaempferide with human serum albumin(HSA) were investigated by cyclic voltammetry. A differential pulse voltammetric method has been proposed for the determination of the HSA with kaempferide. In pH 4. 5 phosphate buffer solutions (PBS) kaempferide can react with HSA to form an electrochemically non-active supermolecular complex at activated GCE,resulting in the decrease of oxidation peak (at 0. 468 V) without the change of peak potential. The decrease of the peak current is proportional to HSA concentration in the range of 0. 05 ～ 0. 25 mg/L. The limit of the detection is 0. 008 mg/L. This method can be applied to the determination of HSA sample.

Keywords

kaempferide, protein, voltammetry

DOI Link

https://doi.org/10.61558/2993-074X.3350

Publication Date

2010-05-28

Online Available Date

2010-05-28

Revised Date

2009-12-22

Received Date

2009-10-16

Recommended Citation

Li-qing LIN, Xin-hua LIN, Jing-hua CHEN, Li-ying HUANG, Yin-huan LIU, Guang-wen LI. Electrochemical Behavior of Kaempferide Interaction with Protein[J]. Journal of Electrochemistry, 2010, 16(2): 233-236.
DOI: 10.61558/2993-074X.3350
Available at: https://jelectrochem.xmu.edu.cn/journal/vol16/iss2/22