Abstract
The linear sweep technique was used to study the electrochemical behavior of epirubicin hydrochloride.The results showed that there was a sensitive reduction peak at-0.34 V(vs.SCE) in pH 6.80 Tris-HCl buffer.When the BSA was added,the value of the reduction peak was reduced deeply.Accordingly,a new electrchemical determintation method of BSA was established.Under the optimum conditions,the decrease of the reduction peak currents of epirubicin hydrochloride were proportional to the concentrations of BSA in the range of 1.0×10-9～1.0×10-6 mol·L-1(R=0.9973).The detection limit of the BSA was 8.05×10-10 mol·L-1.And the binding constant β of the epirubicin hydrochloride and BSA was 3.04×106 L·mol-1 with the ratio of 1.
Keywords
epirubicin hydrochloride, bovine serum albumin(BSA), glassy carbon electrode
Publication Date
2011-02-28
Online Available Date
2011-02-28
Revised Date
2011-02-28
Received Date
2011-02-28
Recommended Citation
Hong-fen CHENG, Yong ZHANG, Jie-qiong SONG, Zeng-qiao WANG.
Interaction of the Epirubicin Hydrochloride with Bovine Serum Albumin and Its Application[J]. Journal of Electrochemistry,
2011
,
17(1): Article 15.
DOI: 10.61558/2993-074X.2820
Available at:
https://jelectrochem.xmu.edu.cn/journal/vol17/iss1/15
References
[1]Ren Haiyang(任海洋),Li Zhiyu(李志裕),Song Bing-sheng(宋炳生).The research progress of anthracycline[J].China Pharmacist(中国药师),2002,5(2):115-116.
[2]María Sereno,Antonella Brunello,Alberto Chiappori,etal.Cardiac toxicity:old and new issues in anti-cancerdrugs[J].Clin Transl Oncol,2008,10:35-46.
[3]National Acopoeia Committee(国家药典委员会).ThePeople's Republic of China Pharmacopoeia[M].Bei-jing:Chemical Industry Press(化学工业出版社),2000,807-809.
[4]Liang Yanqiu(梁彦秋),Zang Shuliang(臧树良),ZhaoXue(赵雪).Interaction of ceftazidime with bovine ser-um album[J].Chinese Journal of Spectroscopy Labora-tory(光谱实验室),2009,26(6):1638-1642.
[5]Zhang Guowen,Zhao Nan,Hu Xing,et al.Interaction ofalpinetin with bovine serum albumin:Probing of themechanismand binding site by spectroscopic methods[J].Spectrochimica Acta Part A,2010,76:410-417.
[6]Zhao Xiaohui(赵小辉),Huang Chengzhi(黄承志).Astudy on the interaction of isamin blue with BSA by res-onance light scattering measurements[J].Journal ofSouthwest China Normal University(西南师范大学学报),2006,31(3):70-75.
[7]Peng Zhen(彭贞),Cheng Lehua(程乐华),Lu Guang-han(陆光汉).Electrochemical study of rhodamine Binteracting with bovine serum albumin(BSA)[J].Jour-nal of Analytical Science(分析科学学报),2006,22(3):318-320.
[8]Yang Pin(杨频).Introduction to bioinorganic chemistry[M].Xi'an:Xi'an Jiaotong University Press(西安交通大学出版社),1991.
