•  
  •  
 

Corresponding Author

Jian-hui JIANG

Abstract

Nucleic acid as the carrier of genetic information and the functional molecules for molecular biology and bioanalytical chemistry has attracted increasing interest in electrochemical analysis. This review presents a brief outline of some electrochemical analytical assays based on molecular recognition of nucleic acids. Most of these methods are focused on the detection of nucleic acid sequence, genetic mutation and nucleic acids as functional molecules.

Graphical Abstract

Publication Date

2011-08-28

Online Available Date

2011-08-09

Revised Date

2011-08-01

Received Date

2011-06-07

References

[1] Fan C H, Plaxco K W, Heeger A J. Electrochemical interrogation of conformational changes as a reagentless method for the sequence-specific detection of DNA[J]. PNAS, 2003, 100: 9134-9137.

[2] Xiao Y, Lubin A A, Baker B R, et al. Single-step electronic detection of femtomolar DNA by target-induced strand displacement in an electrode-bound duplex[J]. PNAS , 2006, 103 (45): 16677-16680.

[3] Zhang Y L, Wang Y, Wang H B, et al. Electrochemical DNA biosensor based on the proximity-dependent surface hybridization assay[J]. Anal Chem, 2009, 81: 1982-1987.

[4] Zhang J, Song S P, Zhang L Y, et al. Sequence-specific detection of femtomolar DNA via a chronocoulometric DNA sensor (CDS): effects of nanoparticle-mediated amplification and nanoscale control of DNA assembly at electrodes[J]. J Am Chem Soc, 2006, 128: 8575-8580.

[5] Patolsky F, Lichtenstein A, Willner I. Highly sensitive amplified electronic detection of DNA by biocatalyzed precipitation of an insoluble production to electrodes[J]. Chem Eur J, 2003, 9: 1137-1145.

[6] Mao X, Jiang J H, Xu X M, et al. Enzymatic ampli?cation detection of DNA based on “molecular beacon” biosensors[J]. Biosens Bioelectron, 2008, 23: 1555-1561.

[7] Kruglyak L, Nickerson D A. Variation is the spice of life[J]. Nat Genet, 2001, 27: 234-236.

[8] Sachidanandam R, Weissman D, Schmidt S C, et al. A map of human genomesequence variation containing 1.42 million single nucleotide polymorphisms[J]. Nature, 2001, 409: 928–933.

[9] Venter J C, Adams M D, Myers E W, et al. The sequence of the human genome[J]. Science, 2001, 291: 1304–1351.

[10] Wu Z S, Jiang J H, Shen G L, et al. Highly sensitive DNA detection and point mutation identification: an electrochemical approach based on the combined use of ligase and reverse molecular beacon[J]. Hum Mutat, 2007, 28: 630-637.

[11] Huang Y, Zhang Y L, Xu X M, et al. Highly speci?c and sensitive electrochemical genotyping via gap ligation reaction and surface hybridization detection[J]. J Am Chem Soc, 2009, 131: 2478-2480.

[12] Zhang S B, Wu Z S, Shen G L, et al. A label-free strategy for SNP detection with high ?delity and sensitivity based on ligation-rolling circle ampli?cation and intercalating of methylene blue[J]. Biosens Bioelectron, 2009, 24: 3201-3207.

[13] Feng K J, Zhao J J, Wu Z S, et al. High-sensitive electrochemical detection of point mutation based on polymerization-induced enzymatic ampli?cation[J]. Biosens Bioelectron, 2011, 26: 3187-3191.

[14] Hu R, Wu Z S, Zhang S B, et al. Robust electrochemical system for screening single nucleotide polymorphisms[J]. Chem Commun, 2011, 47: 1294-1296.

[15] Chen H, Liu X J, Liu Y L, et al. Electro- chemical scanning of DNA point mutations via MutS protein-mediated mismatch recognition[J]. Biosens Bioelectron, 2009, 24: 1955-1961.

[16] Ellington A D, Szostak J W. In vitro selection of RNA molecules that bind specific ligands[J]. Nature, 1990, 346: 818-822.

[17] Tuerk C, Gold L. Systematic evolution of ligands by exponential enrichment RNA ligands to bacteriophage T4 DNA polymerase[J]. Science, 1990, 249: 505-510.

[18] Xiao Y, Lubin A A, Heeger A J, et al. Label-free electronic detection of thrombin in blood serum by using an aptamer-based sensor[J]. Angew Chem Int Ed, 2005, 44: 5456-5459.

[19] Wu Z S, Zheng F, Shen G L, et al. A hairpin aptamer-based electrochemical biosensing platform for the sensitive detection of proteins[J]. Biomaterials, 2009, 30: 2950-2955.

[20] Wu Z S, Chen C R, Shen G L, et al. Reversible electronic nanoswitch based on DNA G-quadruplex conformation: a platform for single-step, reagentless potassium detection[J]. Biomaterials, 2008, 29: 2689–2696.

[21] Zhang S B, Hu R, Hu P, et al. Blank peak current-suppressed electrochemical aptameric sensing platform for highly sensitive signal-on detection of small molecule[J]. Nueleic Acids Res, 2010, 38: e185.

[22] He J L, Yang Y F, Shen G L, et al. Electrochemical aptameric sensor based on the Klenow fragment polymerase reaction for cocaine detection[J]. Biosens Bioelectron, 2011, 26: 4222-4226.

[23] Wu Z S, Guo M M, Zhang S B, et al. Reusable electrochemical sensing platform for highly sensitive detection of small molecules based on structure-switching signaling aptamers[J]. Anal Chem, 2007, 79: 2933-2939.

[24] Zhang Y L, Huang Y, Jiang J H, et al. Electrochemical aptasensor based on proximity-dependent surface hybridization assay for single-step, reusable, sensitive protein detection[J]. J Am Chem Soc, 2007, 129: 15448-15449.

[25] Zhou L, Ou L J, Chu X, et al. Aptamer-based rolling circle amplification: a platform for electrochemical detection of protein[J]. Anal Chem, 2007, 79: 7492-7500.

[26] Gong H, Li X H. Y-type, C-rich DNA probe for electrochemical detection of silver ion and cysteine[J]. Analyst, 2011, 136: 2242-2246.

[27] Ono A, Cao S Q, Togashi H, et al. Specific interactions between silver(I) ions and cytosine-cytosine pairs in DNA duplexes[J]. Chem Commun, 2008: 4825-4827.

[28] Miyake Y, Togashi H, Tashiro M, et al. MercuryII-mediated formation of thymine HgII thymine base pairs in DNA duplexes[J]. J Am Chem Soc, 2006, 128: 2172-2173.

[29] Tanaka Y, Oda S, Yamaguchi H, et al. 15N-15N J-coupling across HgII: direct observation of HgII-mediated T-T base pairs in a DNA duplex[J]. J Am Chem Soc, 2007, 129: 244-245.

[30] Liu S J, Nie H G, Jiang J H, et al. Electrochemical sensor for mercury(II) based on conformational switch mediated by interstrand cooperative coordination[J]. Anal Chem, 2009, 81: 5724-5730.

[31] Wu D H, Zhang Q, Chu X, et al. Ultrasensitive electrochemical sensor for mercury(II) based on target-induced structure-switching DNA[J]. Biosens Bioelectron, 2010, 25: 1025-1031.

[32] Kong R M, Zhang X B, Zhang L L, et al. An ultrasensitive electrochemical ‘‘turn-on’’ label-free biosensor for Hg2+ with AuNP-functionalized reporter DNA as a signal ampli?er[J]. Chem Commun, 2009: 5633-5635.

[33] Zhang Z P, Tang A M, Liao S Z, et al. Oligonucleotide probes applied for sensitive enzyme-ampli?ed electrochemical assay of mercury(II) ions[J]. Biosens Bioelectron, 2011, 26: 3320-3324.

[34] Wu Z, Zhen Z, Jiang J H, et al. Terminal protection of small-molecule-linked DNA for sensitive electrochemical detection of protein binding via selective carbon nanotube assembly[J]. J Am Chem Soc, 2009, 131: 12325-12332.

Share

COinS
 
 

To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.