Electrocatalytic Study of Pseudomonas Aeruginosa BTE-1 Strain

Authors

Ping ZHOU, Jiangsu provincial Key Laboratory of Environmental Materials and Engineering, and Department of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 225002, Jiangsu,China;
En-Ren ZHANG, Jiangsu provincial Key Laboratory of Environmental Materials and Engineering, and Department of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 225002, Jiangsu,China;
Li ZHOU, Jiangsu provincial Key Laboratory of Environmental Materials and Engineering, and Department of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 225002, Jiangsu,China;
Guo-Wang DIAO, Jiangsu provincial Key Laboratory of Environmental Materials and Engineering, and Department of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 225002, Jiangsu,China;
Jun-Le NIU, Jiangsu provincial Key Laboratory of Environmental Materials and Engineering, and Department of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 225002, Jiangsu,China;

Corresponding Author

En-Ren ZHANG(erzhang@yzu.edu.cn)

Abstract

The aim of the present study is to investigate the electrocatalytic activity of electricity-producing Pseudomonas aeruginosa BTE-1 strain under anaerobic conditions. Pseudomonas aeruginosa BTE-1 was inoculated into anaerobic three-electrode electrochemical cells, and the electrocatalytic activity was measured at poised potentials. HPLC and cyclic voltammetry were used to detect potential electron mediators in solutions. Experimental results showed that no detectable pyocyanine was excreted by P. aeruginosa BTE-1 strain in the anaerobic electrochemcial cells, and P. aeruginosa BTE-1 exhibited direct electrocatalytic activity through the formation of biofilm on the electrode surface which was induced by the electron transfer from the cells of P. aeruginosa BTE-1 to the electrode at poised potentials. Suitable potential for biofilm formation was found to be 0.2 V (vs. SCE), and more positive potentials would lead to a potential harm to P. aeruginosa BTE-1 cells. At room temperature, the electrocatalytic activity of the P. aeruginosa BTE-1 biofilm could be enhanced by increasing temperature, however, the temperatures higher than 60 °C reduced the electrocatalytic activity of the biofilms quickly. Cyclic voltammetry analysis indicated that P. aeruginosa BTE-1 biofilms formed under anaerobic conditions exhibited an electrochemical catalytic wave in the potential range of -0.4 V to -0.2 V (vs. SCE), similar to that observed with a typical electricity-generating strain, Geobacter sulfurreducens. Organic substrates, such as compounds in yeast and glucose, could be oxidized through the catalysis of P. aeruginosa BTE-1 biofilms, whereas acetate could not be catalyzed to oxidize.

Graphical Abstract

Keywords

Pseudomonas aeruginosa BTE-1;, biofilm, electrocatalysis

DOI Link

https://doi.org/10.61558/2993-074X.2883

Publication Date

2012-02-28

Online Available Date

2011-10-31

Revised Date

2011-10-04

Received Date

2011-09-13

Recommended Citation

Ping ZHOU, En-Ren ZHANG, Li ZHOU, Guo-Wang DIAO, Jun-Le NIU. Electrocatalytic Study of Pseudomonas Aeruginosa BTE-1 Strain[J]. Journal of Electrochemistry, 2012 , 18(1): Article 12.
DOI: 10.61558/2993-074X.2883
Available at: https://jelectrochem.xmu.edu.cn/journal/vol18/iss1/12

References

[1] Logan B E, Regan J M. Electricity-producing bacterial communities in microbial fuel cells [J]. Trends Microbiol, 2006, 14(12): 512-518.

[2] Luo Y, Zhang R J, Liu G, et al. Electricity generation from indole and microbial community analysis in the microbial fuel cell [J]. J Hazard Mater, 2010, 176(24): 759-764.

[3] Lovley D E. Bug juice: harvesting electricity with microorganisms [J]. Nat Rev Microbiol, 2006, 4(7): 497-508.

[4] Dumas C, Mollica A, Féron D, et al. Checking graphite and stainless anodes with an experimental model of marine microbial fuel cell [J]. Bioresource Technology, 2008, 99(18): 8887–8894.

[5] Jiang J Q(姜珺秋), Zhao Q L(赵庆良), Wei L L(魏亮亮), et al. Extracellular biological organic matters in microbial fuel cell using sewage sludge as fuel [J]. Water Res, 2010, 44(7): 2163-2170.

[6] Bond D R, Srikanth S, Marsili E, et al. Electrochemical Characterization of Geobacter sulfurreducens Cells Immobilized on Graphite Paper Electrodes [J]. Biotechnol Bioeng, 2008, 99(5): 1065-1073.

[7] Marsili E, Rollefson J B, Baron D B, et al. Microbial Bio?lm Voltammetry: Direct Electrochemical Characterization of Catalytic Electrode-Attached Bio?lms [J]. Appl Environ Microb, 2008, 74(23): 7329-7337.

[8] Cao X X (曹效鑫), Fan M Z (范明志), Liang P(梁鹏), et al. Effects of anode potential on the electricity generation performance of Geobacter Sulfurreducens [J]. Chem J Chinese Universities(高等学校化学学报), 2009, 30(5): 983-987.

[9] Niu Z X(牛钟相), Li Y L (李雅林). The Study Progress of Pseudomonas aeruginosa Diseases [J]. Progress in Veterinary Medicine(动物医学进展), 2003, 24 (1): 16-18.

[10] Iversen B G, Brants?ter A B, Aavitsland P. Nationwide study of invasive Pseudomonas aeruginosa infection in Norway: Importance of underlying disease [J]. J Infection, 2008, 57(2): 139-146.

[11] Coenye T, Nelis H J. In vitro and in vivo model systems to study microbial bio?lm formation [J]. Microbiol METH, 2010, 83(2): 89-105.

[12] Rabaey K, Boon N, Hofte M, et al. Microbial Phenazine Production Enhances Electron Transfer in Biofuel Cells [J]. Environ Sci Technol, 2005, 39(9): 3401-3408.

[13] Venkataraman A, Rosenbaum M, Halitschke R, et al. Quorum sensing regulates electric current generation of
Pseudomonas aeruginosa PA14 in bioelectrochemical systems [J]. Electrochem Commun, 2010, 12: 459-462.

[14] Shuang C D(双陈冬), Zhang E R(张恩仁), Diao G W(刁国旺), et al. Electricity generation in microbial fuel
cells catalyze by mixed culture and pure culture[J]. Electrochemictry(电化学), 2008, 14(3): 313-318.

[15] Zhan Y L(詹亚力), Wang Q (王琴), Zhang P P(张佩佩), et al. Investigation on influence factors and mechanism of microbial fuel cell [J]. Chem J Chinese Universities(高等学校化学学报), 2008 , 29(1): 144-14.

[16] Lloyd J R, Leang C, Lovley D R, et al. Biochemical and genetic characterization of PpcA, a periplasmic c-type cytochrome in Geobacter sulfurreducens [J]. Biochem J, 2003, 369(1): 153-161.

[17] Pessanha M, Londer Y Y, Long W C, et al. Redox characterization of Geobacter sulfurreducens cytochrome c(7): Physiological relevance of the conserved residue F15 probed by site-speci?c mutagenesis [J]. Biochem, 2004, 43(30): 9909- 9917.

[18] Magnuson T S, Isoyama N, Lovley D R, et al. Isolation, characterization, and genesequence analysis of a membrane associated 89 kDa Fe(III) reducing cyotchrome from Geobacter sulfurreducens [J]. Biochem J, 2001, 359: 147-152.