Electrochemical Immunosensor Based on Polycalconcarboxylic Acid Modified Electrode for the Determination of Alpha-Fetoprotein

Authors

Xiao LIN, Department of Pharmaceutical Analysis, Faculty of Pharmacy, Fujian Medical University, Fuzhou 350004, China;
Shao-Huang WENG, Department of Pharmaceutical Analysis, Faculty of Pharmacy, Fujian Medical University, Fuzhou 350004, China;
Jian-Zhang ZHOU, State Key Laboratory of Physical Chemistry of the Solid Surface and Department of Chemistry, College of Chemistry and Chemical Engeering, Xiamen University, Xiamen 361005, Fujian, China
Ai-Lin LIU, Department of Pharmaceutical Analysis, Faculty of Pharmacy, Fujian Medical University, Fuzhou 350004, China;
Xin-Hua LIN, Department of Pharmaceutical Analysis, Faculty of Pharmacy, Fujian Medical University, Fuzhou 350004, China;
Yong-Ji YOU, Department of Pharmaceutical Analysis, Faculty of Pharmacy, Fujian Medical University, Fuzhou 350004, China;

Corresponding Author

Xin-Hua LIN(xinhua63@163.com);Yong-Ji YOU

Abstract

A highly sensitive electrochemical immunosensor based on novel electropolymerized polycalconcarboxylic acid modified electrode (poly-CCA/GC) has been fabricated for the detection of alpha-fetoprotein (AFP). The differences of the poly-CCA/GC electrodes before and after the immobilization of AFP antibody were characterized via scanning electron microscopy (SEM) and electrochemical impedance spectroscopy (EIS). The immobilization of antibody/antigen/antibody-HRP sandwich was formed on the poly-CCA modified electrode for the determination of special AFP concentrations. The electrochemical signals generated from the HRP which catalyzed the TMB substrate were measured. Experiment results indicated that the developed immunosensor showed a good sensitivity and precision.

Graphical Abstract

Keywords

polycalconcarboxylic acid modified electrode, alpha-fetoprotein, immunosensor

DOI Link

https://doi.org/10.61558/2993-074X.2933

Publication Date

2012-08-28

Online Available Date

2012-02-20

Revised Date

2012-02-13

Received Date

2012-01-10

Recommended Citation

Xiao LIN, Shao-Huang WENG, Jian-Zhang ZHOU, Ai-Lin LIU, Xin-Hua LIN, Yong-Ji YOU. Electrochemical Immunosensor Based on Polycalconcarboxylic Acid Modified Electrode for the Determination of Alpha-Fetoprotein[J]. Journal of Electrochemistry, 2012 , 18(4): Article 16.
DOI: 10.61558/2993-074X.2933
Available at: https://jelectrochem.xmu.edu.cn/journal/vol18/iss4/16

References

[1] Yin Y M, Cao Y, Xu Y Y, et al. Colorimetric immunoassay for detection of tumor markers[J]. International Journal of Molecular Sciences, 2010, 11(12): 5078-5095.

[2] Wang J, Cao Y, Xu Y Y, et al. Colorimetric multiplexed immunoassay for sequential detection of tumor markers[J]. Biosensors and Bioelectronics, 2009, 25(2): 532-536.

[3] Wu J, Fu Z F, Yan F, et al. Biomedical and clinical applications of immunoassays and immunosensors for tumor markers[J]. Trends in Analytical Chemistry, 2007, 26(7): 679-688.

[4] Liu G D, Lin Y H. Nanomaterial labels in electrochemical immunosensors and immunoassays[J]. Talanta, 2007, 74(3): 308-317.

[5] Xu S J, Liu Y, Wang T H, et al. Positive potential operation of a cathodic electrogenerated chemiluminescence immunosensor basedon luminol and graphene for cancer biomarker detection[J]. Analytical Chemistry, 2011, 83(10): 3817-3823.

[6] Wang S J, Harris E, Shi J, et al. Electrogenerated chemiluminescence determination of C-reactive protein with carboxyl CdSe/ZnS core/shell quantum dots[J]. Physical Chemistry Chemical Physics, 2010, 12(34): 10073-10080.

[7] Lai G S, Wu J, Ju H X, et al. Streptavidin-functionalized silver-nanoparticle-enriched carbon nanotube tag for ultrasensitive multiplexed detection of tumor markers[J]. Advanced Functional Materials, 2011, 21(15): 2938-2943.

[8] Liu A L, Zhang S B, Chen W, et al. Simultaneous voltammetric determination of norepinephrine, ascorbic acid and uric acid on polycalconcarboxylic acid modified glassy carbon electrode[J]. Biosensors and Bioelectronics, 2008, 23(10): 1488-1495.

[9] Weng S H, Lin Z H, Zhang Y, et al. Facile synthesis of SBA-15/polyaniline nanocomposites with high electrochemical activity under neutral and acidic conditions[J], Reactive & Functional Polymers, 2009, 69(2): 130-136.

[10] Li R(李容), Xiong J(熊健), Zhu W Q(朱伟琼), et al. Electrochemical behaviors of hydroquinone on Polypyrrole/STAB-NaMMT/GC electrode[J]. Journal of Electrochemistry(电化学)，2011, 17(4): 453-457.

[11] Wang Z Y, Liu L, Xu Y Y, et al. Simulation and assay of protein biotinylation with electrochemical technique[J], Biosensors and Bioelectronics, 2011, 26(11): 4610-4613.

[12] Wang J, Cao Y, Li Y, et al. Electrochemical strategy for detection of phosphorylation based on enzyme-linked electrocatalysis[J], Journal of Electroanalytical Chemistry, 2011, 656(SI): 274-278.

[13] Weng S H, Lin Z H, Chen L X, et al. Electrochemical synthesis and optical properties of helical polyaniline nanofibers[J]. Electrochimica Acta, 2010, 55(8): 2727-2733.

[14] Fanj-Bolado P, Gonzalez-Garcia M B, Costa-Garcia A. Amperometric detection in TMB/HRP-based assays[J]. Analytical and Bioanalytical Chemistry, 2004, 382(2): 297-302.