Electrochemical Immunosensor Based on Polycalconcarboxylic Acid Modified Electrode for the Determination of Alpha-Fetoprotein

Authors

Xiao LIN, Department of Pharmaceutical Analysis, Faculty of Pharmacy, Fujian Medical University, Fuzhou 350004, China;
Shao-Huang WENG, Department of Pharmaceutical Analysis, Faculty of Pharmacy, Fujian Medical University, Fuzhou 350004, China;
Jian-Zhang ZHOU, State Key Laboratory of Physical Chemistry of the Solid Surface and Department of Chemistry, College of Chemistry and Chemical Engeering, Xiamen University, Xiamen 361005, Fujian, China
Ai-Lin LIU, Department of Pharmaceutical Analysis, Faculty of Pharmacy, Fujian Medical University, Fuzhou 350004, China;
Xin-Hua LIN, Department of Pharmaceutical Analysis, Faculty of Pharmacy, Fujian Medical University, Fuzhou 350004, China;
Yong-Ji YOU, Department of Pharmaceutical Analysis, Faculty of Pharmacy, Fujian Medical University, Fuzhou 350004, China;

Corresponding Author

Xin-Hua LIN(xinhua63@163.com);Yong-Ji YOU

Abstract

A highly sensitive electrochemical immunosensor based on novel electropolymerized polycalconcarboxylic acid modified electrode (poly-CCA/GC) has been fabricated for the detection of alpha-fetoprotein (AFP). The differences of the poly-CCA/GC electrodes before and after the immobilization of AFP antibody were characterized via scanning electron microscopy (SEM) and electrochemical impedance spectroscopy (EIS). The immobilization of antibody/antigen/antibody-HRP sandwich was formed on the poly-CCA modified electrode for the determination of special AFP concentrations. The electrochemical signals generated from the HRP which catalyzed the TMB substrate were measured. Experiment results indicated that the developed immunosensor showed a good sensitivity and precision.

Graphical Abstract

Keywords

polycalconcarboxylic acid modified electrode, alpha-fetoprotein, immunosensor

DOI Link

https://doi.org/10.61558/2993-074X.2933

Publication Date

2012-08-28

Online Available Date

2012-02-20

Revised Date

2012-02-13

Received Date

2012-01-10

Recommended Citation

Xiao LIN, Shao-Huang WENG, Jian-Zhang ZHOU, Ai-Lin LIU, Xin-Hua LIN, Yong-Ji YOU. Electrochemical Immunosensor Based on Polycalconcarboxylic Acid Modified Electrode for the Determination of Alpha-Fetoprotein[J]. Journal of Electrochemistry, 2012 , 18(4): Article 16.
DOI: 10.61558/2993-074X.2933
Available at: https://jelectrochem.xmu.edu.cn/journal/vol18/iss4/16