Electrocatalytic Activities of Mb/AuNPs/MWNTs/GC Electrode for Hydrogen Peroxide Reduction

Authors

Zheng-Juan HUANG, Department of Pharmaceutical Analysis of the Fujian Medical University，Fuzhou 350004，China；
Hua-Ping PENG, Department of Pharmaceutical Analysis of the Fujian Medical University，Fuzhou 350004，China；
Dai-Jun CHA, Institute for Advanced Study of Nanchang University，Nanchang 330031，China；
Ai-Lin LIU, Department of Pharmaceutical Analysis of the Fujian Medical University，Fuzhou 350004，China；
Wei CHEN, Department of Pharmaceutical Analysis of the Fujian Medical University，Fuzhou 350004，China；
Xin-Hua LIN, Department of Pharmaceutical Analysis of the Fujian Medical University，Fuzhou 350004，China；
Yong-Ji YOU, Fujian Provincial Institute for Drug Control，Fuzhou 350001，China

Corresponding Author

Hua-Ping PENG(phpfjmu@126.com);Yong-Ji YOU

Abstract

A novel matrix, multiwalled carbon nanotubes supported Au nanoparticles composite nanomaterial (AuNPs/MWNTs), for immobilization of protein and biosensing was designed using a simple and effective one-step in situ synthesis route. Using myoglobin (Mb) as a model, the direct electrochemistry of the immobilized proteins on the AuNPs/MWNTs composite was studied. The results showed that the AuNPs/MWNTs composite can maintain the bioactivity and facilitate the direct electrochemistry of Mb in the Mb/AuNPs/MWNTs/GC electrode. Based on the direct electron transfer of the immobilized Mb, the protein electrode exhibited excellent electrocatalytic activity to the reduction of H₂O₂ with a linear range of 1 ~ 138 μmol.L^-1，low detection limit of 0.32 μmol.L^-1 (S/N=3) and a low apparent K_m^app value of 0.143 mmol.L^-1. The simple operation, fast response and well reproducibility of the proposed biosensor indicated its promising application in protein immobilization and preparation of the third generation biosensors.

Graphical Abstract

Keywords

myoglobin, AuNPs/MWNTs composite, direct electrochemistry, biosensor

DOI Link

https://doi.org/10.61558/2993-074X.2934

Publication Date

2012-08-28

Online Available Date

2012-04-15

Revised Date

2012-04-10

Received Date

2012-02-25

Recommended Citation

Zheng-Juan HUANG, Hua-Ping PENG, Dai-Jun CHA, Ai-Lin LIU, Wei CHEN, Xin-Hua LIN, Yong-Ji YOU. Electrocatalytic Activities of Mb/AuNPs/MWNTs/GC Electrode for Hydrogen Peroxide Reduction[J]. Journal of Electrochemistry, 2012 , 18(4): Article 17.
DOI: 10.61558/2993-074X.2934
Available at: https://jelectrochem.xmu.edu.cn/journal/vol18/iss4/17

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