Abstract
A novel matrix, multiwalled carbon nanotubes supported Au nanoparticles composite nanomaterial (AuNPs/MWNTs), for immobilization of protein and biosensing was designed using a simple and effective one-step in situ synthesis route. Using myoglobin (Mb) as a model, the direct electrochemistry of the immobilized proteins on the AuNPs/MWNTs composite was studied. The results showed that the AuNPs/MWNTs composite can maintain the bioactivity and facilitate the direct electrochemistry of Mb in the Mb/AuNPs/MWNTs/GC electrode. Based on the direct electron transfer of the immobilized Mb, the protein electrode exhibited excellent electrocatalytic activity to the reduction of H2O2 with a linear range of 1 ~ 138 μmol.L-1，low detection limit of 0.32 μmol.L-1 (S/N=3) and a low apparent Kmapp value of 0.143 mmol.L-1. The simple operation, fast response and well reproducibility of the proposed biosensor indicated its promising application in protein immobilization and preparation of the third generation biosensors.
Graphical Abstract
Keywords
myoglobin, AuNPs/MWNTs composite, direct electrochemistry, biosensor
Publication Date
2012-08-28
Online Available Date
2012-04-15
Revised Date
2012-04-10
Received Date
2012-02-25
Recommended Citation
Zheng-Juan HUANG, Hua-Ping PENG, Dai-Jun CHA, Ai-Lin LIU, Wei CHEN, Xin-Hua LIN, Yong-Ji YOU.
Electrocatalytic Activities of Mb/AuNPs/MWNTs/GC Electrode for Hydrogen Peroxide Reduction[J]. Journal of Electrochemistry,
2012
,
18(4): Article 17.
DOI: 10.61558/2993-074X.2934
Available at:
https://jelectrochem.xmu.edu.cn/journal/vol18/iss4/17
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