A Study on Electrochemical Oxidation of Reduced Nicotinamide Coenzyme NADH in Organic Media

Authors

Fangzu Yang, Institute of New Energy Material Chemistry,Nankai University,Tianjin 300071;
WuHuihuang WuHuihuang, Dept.of Chem.,State Key Laboratory for Physical Chemistry of Solid Surface,Xiamen University,Xiamen 361005;
XuShukai XuShukai, Dept.of Chem.,State Key Laboratory for Physical Chemistry of Solid Surface,Xiamen University,Xiamen 361005;
ZhouShsomin ZhouShsomin, Dept.of Chem.,State Key Laboratory for Physical Chemistry of Solid Surface,Xiamen University,Xiamen 361005;

Abstract

The behaviour of electrochemical oxidation of the reduced nicotinamide coenzyme NADH on glassy carbon was studied and some kinetic parameters were measured by means of voltammometry in the N-dimethylformamide(DMF)and 1,4-dioxane media respectively. The eletrochemical oxidation of NADH shows completely irriversable both in the two solvents,and the diagnostic experiments support that the process involves a fast chemical step.The oxidation rate and the diffusion coefficient of N ADH in DMP are smaller than those in 1,4-dioxane,but the response current is more stable in DMF. The experimental results are discussed by solvent effects.

Keywords

NADH, Electrochemical oxidation, organic phase enzymology, bioeletrochemistry

DOI Link

https://doi.org/10.61558/2993-074X.1338

Publication Date

1996-08-28

Online Available Date

1996-08-28

Revised Date

1996-08-28

Received Date

1996-08-28

Recommended Citation

Fangzu Yang, WuHuihuang WuHuihuang, XuShukai XuShukai, ZhouShsomin ZhouShsomin. A Study on Electrochemical Oxidation of Reduced Nicotinamide Coenzyme NADH in Organic Media[J]. Journal of Electrochemistry, 1996 , 2(3): 294-298.
DOI: 10.61558/2993-074X.1338
Available at: https://jelectrochem.xmu.edu.cn/journal/vol2/iss3/9