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Corresponding Author

Ai-lin LIU(ailinliu@mail.fjmu.edu.cn);Xin-hua LIN(xinhua63@163.com)

Abstract

A novel platform for the fabrication of glucose biosensor was successfully constructed by entrapping glucose oxidase (GOD) in a ferrocene monocarboxylic acid-aminated Fe3O4 magnetic nanoparticles conjugate (FMC-AFNPs)/chitosan (CS)/multiwall carbon nanotubes (MWNTs) nanocomposite. The formation of FMC-AFNPs could effectively prevent the leakage of ferrocene and retain its electrochemical activity. This GOD/FMC-AFNPs/CS/MWNTs matrix provided a biocompatible microenvironment for retaining the native activity of the immobilized GOD. Moreover, the presence of MWNTs enhanced the charge-transport properties of the composite and facilitated electron transfer between the GOD and the electrode for the electrocatalysis of glucose. Under the optimal conditions the designed biosensor to glucose exhibited a wide and useful linear range of 1.0×10-5 to 6.0×10-3 molL-1 with a low detection limit of 3.2×10-6 molL-1（S/N=3）. The value of was 5.03×10-3 molL-1, indicating that the biosensor possesses higher biological affinity to glucose. Furthermore, the biosensor possesses satisfactory stability and good reproducibility.

Graphical Abstract

Keywords

ferrocene-modified Fe3O4 nanoparticles, carbon nanotubes, chitosan, glucose oxidase, biosensor

Publication Date

2014-02-25

Online Available Date

2014-02-24

Revised Date

2013-01-25

Received Date

2012-11-08

References

[1]Kandimalla V B, Tripathi V S, Ju H X. A conductive ormosil encapsulated with ferrocene conjugate and multiwalled carbon nanotubes for biosensing application[J]. Biomaterials, 2006, 27(7): 1167-1174.

[2]Chen L, Gorski W. Bioinorganic composites for enzyme electrodes[J]. Analytical Chemistry, 2001, 73(13): 2862-2868.

[3]Pandey P C, Upadhyay S, Tiwari I, et al. A novel ferrocene-encapsulated palladium-linked ormosil-based electrocatalytic biosensor. The role of the reactive functional group[J]. Electroanalysis, 2001, 13(18): 1519-1527.

[4]Tsiafoulis C G, Florou A B, Trikalitis P N, et al. Electrochemical study of ferrocene intercalated vanadium pentoxide xerogel/polyvinyl alcohol composite films: Application in the development of amperometric biosensors[J]. Electrochemistry Communications, 2005, 7(7): 781-788.

[5]Liang R P, Fan L X, Huang D M, et al. A Label-free amperometric immunosensor based on redox-active ferrocene-branched chitosan/multiwalled carbon nanotubes conductive composite and gold nanoparticles[J]. Electroanalysis, 2011, 23(3): 719-727.

[6]Liu X Q, Shi L H, Niu W X, et al. Amperometric glucose biosensor based on single-walled carbon nanohorns[J]. Biosensors and Bioelectronics, 2008, 23(12), 1887-1890.

[7]Hu L Z, Han S, Liu Z Y, et al. A versatile strategy for electrochemical detection of hydrogen peroxide as well as related enzymes and substrates based on selective hydrogen peroxide-mediated boronate deprotection[J]. Electrochemistry Communications, 2011, 13(12): 1536-1538.

[8]Lei J P, Ju H X. Signal amplification using functional nanomaterials for biosensing[J]. Chemical Society Reviews, 2012, 41(6): 2122-2134.

[9]Dumitresscu I, Unwin P R, Macpherson J V. Electrochemistry at carbon nanotubes: Perspectives and issues[J]. Chemical Communications, 2009, 46(45): 6886-6901.

[10]Jacobs C B, Peairs M J, Venton B J. Review: Carbon nanotube based electrochemical sensors for biomolecules[J]. Analytica Chimica Acta, 2010, 662(2): 105-127.

[11]Huang Y, Zhao S L, Liu Y M, et al. An amplified single-walled carbon nanotube-mediated chemiluminescence turn-on sensing platform for ultrasensitive DNA detection[J]. Chemical Communications, 2012, 48(75): 9400-9402.

[12]Cruz J, Kawasaki M, Gorski W. Electrode coatings based on chitosan scaffolds[J]. Analytical Chemistry, 2000, 72(4): 680-686.

[13]Benesch J, Tengvall P. Blood protein adsorption onto chitosan[J]. Biomaterials, 2002, 23(12): 2561-2568.

[14]Liang R P, Fan L. X, Wang R, et al. One-step electrochemically deposited nanocomposite film of CS-Fc/MWNTs/GOD for glucose biosensor application[J]. Electroanalysis, 2009, 21(15): 1685-1691.

[15]Massart R. Preparation of aqueous magnetic liquids in alkaline and acidic media[J]. IEEE Transactions on Magnetics, 1981, 17(2): 1247-1248.

[16]Peng H P, Qiu J D, Liang R P. Facile synthesis of Fe3O4@Al2O3 core-shell nanoparticles and their application to the highly specific capture of heme proteins for direct electrochemistry[J]. Biosensors and Bioelectronics, 2011, 26(6): 3005-3011.

[17]Yang C Q, Wang, G, Lu Z Y, et al. Effect of ultrasonic treatment on dispersibility of Fe3O4 nanoparticles and synthesis of multi-core Fe3O4/SiO2 core/shell nanoparticles[J]. Journal of Materials Chemistry, 2005, 15(39): 4252-4257.

[18]Li J, Wang Y B, Qiu J D. Biocomposites of covalently linked glucose oxidase on carbon nanotubes for glucose biosensor[J]. Analytical and Bioanalytical Chemistry, 2005, 383(6) 918-922.

[19]Kamin R A, Wilson G S. Rotating ring-disk enzyme electrode for biocatalysis kinetic studies and characterization of the immobilized enzyme layer[J]. Analytical Chemistry, 1980, 52(8): 1198-1205.

[20]Qiu J D, Peng H P, Liang R P, et al. Facile preparation of magnetic core-shell Fe3O4@Au nanoparticle/myoglobin biofilm for direct electrochemistry[J]. Biosensors and Bioelectronics, 2010, 25(6), 1447-1453.

[21]Wu B Y, Hou S H, Yin F, et al. Amperometric glucose biosensor based on multilayer films via layer-by-layer self-assembly of multi-wall carbon nanotubes, gold nanoparticles and glucose oxidase on the Pt electrode[J]. Biosensors and Bioelectronics, 2007, 22(12): 2854-2860.

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