Abstract
A novel platform for the fabrication of glucose biosensor was successfully constructed by entrapping glucose oxidase (GOD) in a ferrocene monocarboxylic acid-aminated Fe3O4 magnetic nanoparticles conjugate (FMC-AFNPs)/chitosan (CS)/multiwall carbon nanotubes (MWNTs) nanocomposite. The formation of FMC-AFNPs could effectively prevent the leakage of ferrocene and retain its electrochemical activity. This GOD/FMC-AFNPs/CS/MWNTs matrix provided a biocompatible microenvironment for retaining the native activity of the immobilized GOD. Moreover, the presence of MWNTs enhanced the charge-transport properties of the composite and facilitated electron transfer between the GOD and the electrode for the electrocatalysis of glucose. Under the optimal conditions the designed biosensor to glucose exhibited a wide and useful linear range of 1.0×10-5 to 6.0×10-3 molL-1 with a low detection limit of 3.2×10-6 molL-1（S/N=3）. The value of was 5.03×10-3 molL-1, indicating that the biosensor possesses higher biological affinity to glucose. Furthermore, the biosensor possesses satisfactory stability and good reproducibility.
Graphical Abstract
Keywords
ferrocene-modified Fe3O4 nanoparticles, carbon nanotubes, chitosan, glucose oxidase, biosensor
Publication Date
2014-02-25
Online Available Date
2014-02-24
Revised Date
2013-01-25
Received Date
2012-11-08
Recommended Citation
Hua-ping PENG, Dai-Jun ZHA, Wei CHEN, Ai-lin LIU, Xin-hua LIN.
Design and Development of a Novel Glucose Biosensor Based on the Ferrocene-Functionalized Fe3O4 Nanoparticles/Carbon Nanotubes/Chitosan Nanocomposite Film Modified Electrode[J]. Journal of Electrochemistry,
2014
,
20(1): 121108.
DOI: 10.13208/j.electrochem.121108
Available at:
https://jelectrochem.xmu.edu.cn/journal/vol20/iss1/15
Included in
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