Corresponding Author

Ai-lin LIU(ailinliu@mail.fjmu.edu.cn);Xin-hua LIN(xinhua63@163.com)


A novel platform for the fabrication of glucose biosensor was successfully constructed by entrapping glucose oxidase (GOD) in a ferrocene monocarboxylic acid-aminated Fe3O4 magnetic nanoparticles conjugate (FMC-AFNPs)/chitosan (CS)/multiwall carbon nanotubes (MWNTs) nanocomposite. The formation of FMC-AFNPs could effectively prevent the leakage of ferrocene and retain its electrochemical activity. This GOD/FMC-AFNPs/CS/MWNTs matrix provided a biocompatible microenvironment for retaining the native activity of the immobilized GOD. Moreover, the presence of MWNTs enhanced the charge-transport properties of the composite and facilitated electron transfer between the GOD and the electrode for the electrocatalysis of glucose. Under the optimal conditions the designed biosensor to glucose exhibited a wide and useful linear range of 1.0×10-5 to 6.0×10-3 molL-1 with a low detection limit of 3.2×10-6 molL-1(S/N=3). The value of was 5.03×10-3 molL-1, indicating that the biosensor possesses higher biological affinity to glucose. Furthermore, the biosensor possesses satisfactory stability and good reproducibility.

Graphical Abstract


ferrocene-modified Fe3O4 nanoparticles, carbon nanotubes, chitosan, glucose oxidase, biosensor

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