Detection of Glucosamine Hydrochloride by Ion Chromatography with Integrated Pulsed Amperometric Detector

Authors

Yan-qun FAN, Third Institute of Oceanography State Oceanic Administration, Xiamen 361005, Fujian, China;College of Life Science, Xiamen University, Xiamen 361005, Fujian, China;
Qing-yang CHEN, Third Institute of Oceanography State Oceanic Administration, Xiamen 361005, Fujian, China;
Jin-mei XIA, Third Institute of Oceanography State Oceanic Administration, Xiamen 361005, Fujian, China;
Jian-zhong XU, Third Institute of Oceanography State Oceanic Administration, Xiamen 361005, Fujian, China;
Xun XU, Third Institute of Oceanography State Oceanic Administration, Xiamen 361005, Fujian, China;College of Life Science, Xiamen University, Xiamen 361005, Fujian, China;
Chen XU, Third Institute of Oceanography State Oceanic Administration, Xiamen 361005, Fujian, China;Follow

Corresponding Author

Chen XU(xmxuchen@163.com)

Abstract

Factors (integral potential, concentration of NaOH and flow rate) affecting detection of Glucosamine hydrochloride by ion chromatography with integrated pulsed amperometric detector were studied. As the integral potential increased, the detection signal first increased and then decreased, while the detection background and noise increased directly. The suitable integral potential should be 0.1 V. With the concentration of NaOH increased, the retention time descended sharply, while the integral area increased quickly before they are coming back into balance. The number of theoretical plates increased firstly and then decreased. The optimum concentration of NaOH was 50 mmol·L^-1. As the flow rate increased, the retention time and peak area reduced. Thus, the flow rate of 0.6 mL·min^-1 was the suitable one. The transformation-quantity of Glucosamine hydrochloride using ED3000 detector was 1.27% by connecting the two integrated pulsed amperometric detectors, while the total amount was 2.79% by NJPAD detector.

Graphical Abstract

Keywords

integrated pulsed amperometric detector, glucosamine hydrochloride, transformation-quantity

DOI Link

https://doi.org/10.13208/j.electrochem.130620

Publication Date

2014-04-28

Online Available Date

2014-04-17

Revised Date

2013-12-02

Received Date

2013-07-05

Recommended Citation

Yan-qun FAN, Qing-yang CHEN, Jin-mei XIA, Jian-zhong XU, Xun XU, Chen XU. Detection of Glucosamine Hydrochloride by Ion Chromatography with Integrated Pulsed Amperometric Detector[J]. Journal of Electrochemistry, 2014 , 20(2): 164-170.
DOI: 10.13208/j.electrochem.130620
Available at: https://jelectrochem.xmu.edu.cn/journal/vol20/iss2/11