Preparation of a New Laccase Biosensor based on Bimetallic Pt/Au Particles and Chitosan/silica sol-gel Hybrid Films for Determination of Catechol

Authors

Tong-fang LOU, Institute of Environmental and Analytical Sciences, College of Chemistry and Chemical Engineering, Henan University, Kaifeng 475004, Henan, China;
Jian-ying QU, Institute of Environmental and Analytical Sciences, College of Chemistry and Chemical Engineering, Henan University, Kaifeng 475004, Henan, China;Follow
Huan-huan XING, Institute of Environmental and Analytical Sciences, College of Chemistry and Chemical Engineering, Henan University, Kaifeng 475004, Henan, China;
Xue-ping DU, Institute of Environmental and Analytical Sciences, College of Chemistry and Chemical Engineering, Henan University, Kaifeng 475004, Henan, China;
Ying DONG, Institute of Environmental and Analytical Sciences, College of Chemistry and Chemical Engineering, Henan University, Kaifeng 475004, Henan, China;

Corresponding Author

Jian-ying QU(lilyqujy@yahoo.com.cn)

Abstract

A novel laccase (Lac) biosensor was fabricated successfully by means of entrapping Lac into bimetallic Pt/Au particles and chitosan/silica (CS-SiO2) sol-gel hybrid films, and then applied to determine catechol (CC) concentration. The experiment results showed that the biosensor can electrocatalyze the reduction of CC, and the peak current increased linearly with the concentration of CC in the range of 8×10^-7 ~ 1×10^-4 mol·L^-1 (r = 0.9993) with a detection limit of 7.9×10^-8 mol·L^-1 by cyclic voltammetric response. The biosensor exhibited good sensitivity, stability and reproducibility, with a broad linear range for potential applications.

Graphical Abstract

DOI Link

https://doi.org/10.13208/j.electrochem.130809

Publication Date

2014-04-28

Online Available Date

2014-04-17

Revised Date

2013-10-25

Received Date

2013-08-09

Recommended Citation

Tong-fang LOU, Jian-ying QU, Huan-huan XING, Xue-ping DU, Ying DONG. Preparation of a New Laccase Biosensor based on Bimetallic Pt/Au Particles and Chitosan/silica sol-gel Hybrid Films for Determination of Catechol[J]. Journal of Electrochemistry, 2014 , 20(2): 177-183.
DOI: 10.13208/j.electrochem.130809
Available at: https://jelectrochem.xmu.edu.cn/journal/vol20/iss2/13